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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30623 | 3' | -54.9 | NC_006549.1 | + | 50800 | 0.66 | 0.905339 |
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Target: 5'- uGGGUCcuggcggGCCCGCugggccUGUUGCGCCc--- -3' miRNA: 3'- uCUCAGa------CGGGUG------ACAACGCGGuugu -5' |
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| 30623 | 3' | -54.9 | NC_006549.1 | + | 112188 | 0.66 | 0.892099 |
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Target: 5'- -cGGUCUGaaCCCGgaGacGCGCCAACAc -3' miRNA: 3'- ucUCAGAC--GGGUgaCaaCGCGGUUGU- -5' |
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| 30623 | 3' | -54.9 | NC_006549.1 | + | 139182 | 0.67 | 0.846773 |
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Target: 5'- gAGAGUCUGCaggauUugUGgucaUUGCGCCAAa- -3' miRNA: 3'- -UCUCAGACGg----GugAC----AACGCGGUUgu -5' |
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| 30623 | 3' | -54.9 | NC_006549.1 | + | 42378 | 0.68 | 0.838461 |
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Target: 5'- gGGAGUgaGCCgAUUGUuugaaacccugUGCGCCGAa- -3' miRNA: 3'- -UCUCAgaCGGgUGACA-----------ACGCGGUUgu -5' |
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| 30623 | 3' | -54.9 | NC_006549.1 | + | 122624 | 0.7 | 0.725815 |
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Target: 5'- -uGGUCcGCCU-CUGUUGCGCCAGu- -3' miRNA: 3'- ucUCAGaCGGGuGACAACGCGGUUgu -5' |
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| 30623 | 3' | -54.9 | NC_006549.1 | + | 131917 | 1.08 | 0.003028 |
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Target: 5'- cAGAGUCUGCCCACUGUUGCGCCAACAu -3' miRNA: 3'- -UCUCAGACGGGUGACAACGCGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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