Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30624 | 3' | -49.2 | NC_006549.1 | + | 96868 | 0.66 | 0.996462 |
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Target: 5'- -----aUGGUGGCCGCGuACGACGg- -3' miRNA: 3'- uccuaaACCGUCGGCGUuUGUUGUag -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 122559 | 0.66 | 0.995093 |
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Target: 5'- ----gUUGGCuGCCGCAgcAGCGGCGa- -3' miRNA: 3'- uccuaAACCGuCGGCGU--UUGUUGUag -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 78145 | 0.67 | 0.991075 |
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Target: 5'- cGGGUUuuacaaccUGcGCAGgCGCAAAC-GCGUCu -3' miRNA: 3'- uCCUAA--------AC-CGUCgGCGUUUGuUGUAG- -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 60227 | 0.67 | 0.989747 |
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Target: 5'- cAGGGUguaGGCAuCUGUuAGCGGCGUCg -3' miRNA: 3'- -UCCUAaa-CCGUcGGCGuUUGUUGUAG- -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 60457 | 0.68 | 0.984823 |
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Target: 5'- cGGcgUUGGCGGUgugCGCcGAUAGCGUg -3' miRNA: 3'- uCCuaAACCGUCG---GCGuUUGUUGUAg -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 9139 | 0.68 | 0.979963 |
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Target: 5'- uGGGA---GGCgugauGGCCGCAAGCGgcuacgcugcuuauACAUCg -3' miRNA: 3'- -UCCUaaaCCG-----UCGGCGUUUGU--------------UGUAG- -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 17916 | 0.7 | 0.959303 |
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Target: 5'- -----aUGGCAGCCGcCAGAUAACGc- -3' miRNA: 3'- uccuaaACCGUCGGC-GUUUGUUGUag -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 123978 | 0.71 | 0.913494 |
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Target: 5'- ---uUUUGGCggcuacGGCCGCAGACGACGcuUCa -3' miRNA: 3'- uccuAAACCG------UCGGCGUUUGUUGU--AG- -5' |
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| 30624 | 3' | -49.2 | NC_006549.1 | + | 133081 | 1.12 | 0.006626 |
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Target: 5'- gAGGAUUUGGCAGCCGCAAACAACAUCa -3' miRNA: 3'- -UCCUAAACCGUCGGCGUUUGUUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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