Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30626 | 3' | -49.5 | NC_006549.1 | + | 70628 | 0.66 | 0.997838 |
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Target: 5'- gGC-CGUGgcgUugUGCGGuuuGGGCGUCAa -3' miRNA: 3'- -UGaGCAU---GugACGCCuuuCUUGCAGU- -5' |
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| 30626 | 3' | -49.5 | NC_006549.1 | + | 131706 | 0.67 | 0.995018 |
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Target: 5'- uUUCGUACACUcCGGAAAGcgaGGCGggCGg -3' miRNA: 3'- uGAGCAUGUGAcGCCUUUC---UUGCa-GU- -5' |
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| 30626 | 3' | -49.5 | NC_006549.1 | + | 132989 | 0.7 | 0.963873 |
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Target: 5'- -aUCGUACAUgcgcGCGGAAucGAACGUUu -3' miRNA: 3'- ugAGCAUGUGa---CGCCUUu-CUUGCAGu -5' |
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| 30626 | 3' | -49.5 | NC_006549.1 | + | 5190 | 0.7 | 0.952252 |
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Target: 5'- --gCGUGCAUUG-GGGAGGAACGUg- -3' miRNA: 3'- ugaGCAUGUGACgCCUUUCUUGCAgu -5' |
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| 30626 | 3' | -49.5 | NC_006549.1 | + | 56896 | 0.72 | 0.909942 |
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Target: 5'- --gCGgcCGCUGCGGgcAGggUGUCAg -3' miRNA: 3'- ugaGCauGUGACGCCuuUCuuGCAGU- -5' |
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| 30626 | 3' | -49.5 | NC_006549.1 | + | 139737 | 1.09 | 0.011023 |
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Target: 5'- gACUCGUACACUGCGGAAAGAACGUCAu -3' miRNA: 3'- -UGAGCAUGUGACGCCUUUCUUGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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