miRNA: 3'- aCG-GUGUGGgA-AACCACG---CGCUC---------UGC- -5'
miRNA: 3'- aCGgugUGGGAAACCac-GCGCUCUGc -5'
miRNA: 3'- -ACGGUGUGGGAAACCACGCGCUCUGC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30626 | 5' | -56.5 | NC_006549.1 | + | 113845 | 0.66 | 0.879394 |
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Target: 5'- cGCgCACACCgUcUUGGUGCccaGCGAGuuuuuuaucGCGg -3' miRNA: 3'- aCG-GUGUGGgA-AACCACG---CGCUC---------UGC- -5' |
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| 30626 | 5' | -56.5 | NC_006549.1 | + | 102558 | 0.68 | 0.751194 |
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Target: 5'- cGCa--GCCCUUUGGgccCGCGGGGCc -3' miRNA: 3'- aCGgugUGGGAAACCac-GCGCUCUGc -5' |
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| 30626 | 5' | -56.5 | NC_006549.1 | + | 139699 | 1.09 | 0.002188 |
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Target: 5'- uUGCCACACCCUUUGGUGCGCGAGACGa -3' miRNA: 3'- -ACGGUGUGGGAAACCACGCGCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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