miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30627 3' -44.5 NC_006549.1 + 681 0.66 0.999994
Target:  5'- gGUCUGUcucGGAAAUCGuCGAGUAcGAAACa -3'
miRNA:   3'- -CGGACA---CUUUUGGC-GUUUAUcCUUUG- -5'
30627 3' -44.5 NC_006549.1 + 57187 0.66 0.999991
Target:  5'- aGCCUGUccugaGAGAGCUGCGc--GGGGuuCa -3'
miRNA:   3'- -CGGACA-----CUUUUGGCGUuuaUCCUuuG- -5'
30627 3' -44.5 NC_006549.1 + 33087 0.67 0.999987
Target:  5'- cGCCacgGUacAAACCGCGu---GGAAACg -3'
miRNA:   3'- -CGGa--CAcuUUUGGCGUuuauCCUUUG- -5'
30627 3' -44.5 NC_006549.1 + 51519 0.67 0.999987
Target:  5'- aCCUGUGGAaggacacgGACCGC--GUGGuGAGAa -3'
miRNA:   3'- cGGACACUU--------UUGGCGuuUAUC-CUUUg -5'
30627 3' -44.5 NC_006549.1 + 118646 0.67 0.999975
Target:  5'- -aCUGaUGGAAAUgGCAAAggGGGAGAUu -3'
miRNA:   3'- cgGAC-ACUUUUGgCGUUUa-UCCUUUG- -5'
30627 3' -44.5 NC_006549.1 + 108827 0.67 0.999975
Target:  5'- cGCUuuuugaUGUGAAAAUCGCAAuuacguAUAGGcguaAAACa -3'
miRNA:   3'- -CGG------ACACUUUUGGCGUU------UAUCC----UUUG- -5'
30627 3' -44.5 NC_006549.1 + 127659 0.68 0.999886
Target:  5'- cGCCUGUGAugaGAACCGUGAugaacGcGAAAUc -3'
miRNA:   3'- -CGGACACU---UUUGGCGUUuau--C-CUUUG- -5'
30627 3' -44.5 NC_006549.1 + 10708 0.69 0.999748
Target:  5'- aCCgc--GAAACCGCGAcuccGUGGGAAGCu -3'
miRNA:   3'- cGGacacUUUUGGCGUU----UAUCCUUUG- -5'
30627 3' -44.5 NC_006549.1 + 68852 0.7 0.998991
Target:  5'- cCCUGUGGAuACCgGCGGgcaccgucaucAUAGGAAAg -3'
miRNA:   3'- cGGACACUUuUGG-CGUU-----------UAUCCUUUg -5'
30627 3' -44.5 NC_006549.1 + 29281 0.71 0.997317
Target:  5'- cGCCgUGgccacgGGAGucGCCGCGAgcguucucAUAGGAAACg -3'
miRNA:   3'- -CGG-ACa-----CUUU--UGGCGUU--------UAUCCUUUG- -5'
30627 3' -44.5 NC_006549.1 + 137452 0.71 0.997317
Target:  5'- cGCUUGUGAGGuccucuGCCGCGGugAGGAu-- -3'
miRNA:   3'- -CGGACACUUU------UGGCGUUuaUCCUuug -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.