Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30628 | 5' | -60.4 | NC_006549.1 | + | 14612 | 0.66 | 0.712412 |
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Target: 5'- gGGCCCCCACACCAcccccacGCcCGCCc---- -3' miRNA: 3'- aUUGGGGGUGUGGU-------CGcGCGGcugcu -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 139703 | 0.66 | 0.66388 |
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Target: 5'- cAGCUUgCCACACCcuuuGGUGCGCgaGACGAu -3' miRNA: 3'- aUUGGG-GGUGUGG----UCGCGCGg-CUGCU- -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 102231 | 0.68 | 0.563935 |
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Target: 5'- gAACCCCUGgGguucCCGGCGCGCU-ACGAu -3' miRNA: 3'- aUUGGGGGUgU----GGUCGCGCGGcUGCU- -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 89943 | 0.69 | 0.505715 |
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Target: 5'- aAACCCUCAaGgCGGCGacaGCUGACGAa -3' miRNA: 3'- aUUGGGGGUgUgGUCGCg--CGGCUGCU- -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 102465 | 0.7 | 0.477572 |
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Target: 5'- gGACUCCUgggGCGCCAGCuGgGCCGGgGGg -3' miRNA: 3'- aUUGGGGG---UGUGGUCG-CgCGGCUgCU- -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 86166 | 0.7 | 0.43248 |
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Target: 5'- cGACgCCUCACACCAauCGC-CCGACGAg -3' miRNA: 3'- aUUG-GGGGUGUGGUc-GCGcGGCUGCU- -5' |
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| 30628 | 5' | -60.4 | NC_006549.1 | + | 6181 | 0.71 | 0.398251 |
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Target: 5'- cUGugCCgCACACCccGGCgGCGCUGGCGGc -3' miRNA: 3'- -AUugGGgGUGUGG--UCG-CGCGGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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