Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30629 | 3' | -58.8 | NC_006549.1 | + | 57239 | 0.66 | 0.771565 |
Target: 5'- gGGUucCGCGGUGGUcagcaGGGCccucGCUACCUCGg -3' miRNA: 3'- aCUA--GCGCCACUA-----CUCG----CGGUGGGGC- -5' |
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30629 | 3' | -58.8 | NC_006549.1 | + | 52147 | 0.67 | 0.71431 |
Target: 5'- aUGcUCGCGuuUGAUuacGUGCCGCCCCGg -3' miRNA: 3'- -ACuAGCGCc-ACUAcu-CGCGGUGGGGC- -5' |
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30629 | 3' | -58.8 | NC_006549.1 | + | 73519 | 0.69 | 0.604291 |
Target: 5'- aGAUUGUGGaUGAUG-GUGCCAaaUCCCa -3' miRNA: 3'- aCUAGCGCC-ACUACuCGCGGU--GGGGc -5' |
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30629 | 3' | -58.8 | NC_006549.1 | + | 97750 | 0.72 | 0.459283 |
Target: 5'- aGA-CGCGGUGcUGGGCGCgCAUCUCa -3' miRNA: 3'- aCUaGCGCCACuACUCGCG-GUGGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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