miRNA: 3'- -GCGCCgCAUGCGguua-GGGU--------------UUUGCG- -5'
miRNA: 3'- gCGCCGca--UGCGGuuAGGGU----UUugCG- -5'
miRNA: 3'- -GCGCCG---------CAUgCGGUuaGGGUUuu---GCG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30629 | 5' | -52.7 | NC_006549.1 | + | 109385 | 0.66 | 0.980746 |
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Target: 5'- aCGCGGgGUGCGUauaacCCCAcgugccuccuauaaAAGCGUu -3' miRNA: 3'- -GCGCCgCAUGCGguua-GGGU--------------UUUGCG- -5' |
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| 30629 | 5' | -52.7 | NC_006549.1 | + | 78027 | 0.66 | 0.981385 |
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Target: 5'- uGCGGUagccGCGCC--UCCCGacggGAugGCa -3' miRNA: 3'- gCGCCGca--UGCGGuuAGGGU----UUugCG- -5' |
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| 30629 | 5' | -52.7 | NC_006549.1 | + | 46012 | 0.66 | 0.981385 |
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Target: 5'- aCGCGGCcuuuagagaGUGgGCCAcgCUCAAcugccCGCa -3' miRNA: 3'- -GCGCCG---------CAUgCGGUuaGGGUUuu---GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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