miRNA: 3'- gCGCCGCauGCGGU--------UAGGGuUUUGCg -5'
miRNA: 3'- gcgCCGCAUGCGGUU----AGg--GUUUUGCg -5'
miRNA: 3'- gCGCCgCAUGCGGUuAGGguUUug-CG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30629 | 5' | -52.7 | NC_006549.1 | + | 129812 | 0.68 | 0.931269 |
|
Target: 5'- aGCGGUGgcCGUCAaggaacacAUCCCuAAGCGg -3' miRNA: 3'- gCGCCGCauGCGGU--------UAGGGuUUUGCg -5' |
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| 30629 | 5' | -52.7 | NC_006549.1 | + | 130193 | 0.69 | 0.902053 |
|
Target: 5'- --aGGCGUGCGCCAGaugcUCggaCGAAACGg -3' miRNA: 3'- gcgCCGCAUGCGGUU----AGg--GUUUUGCg -5' |
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| 30629 | 5' | -52.7 | NC_006549.1 | + | 134747 | 0.7 | 0.851136 |
|
Target: 5'- aGCGGgGUGCGCCA-UCCguAGuuaGCc -3' miRNA: 3'- gCGCCgCAUGCGGUuAGGguUUug-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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