Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30631 | 5' | -57.5 | NC_006549.1 | + | 100223 | 0.66 | 0.809306 |
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Target: 5'- uGCCCUcuggcUGGGGACCucGGGCUUCggggGAg -3' miRNA: 3'- -CGGGA-----ACUCCUGGccCUCGAGGaa--CU- -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 102436 | 0.67 | 0.773132 |
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Target: 5'- gGUCCagGAGGGCCGGG-GUUCaCgccUGAg -3' miRNA: 3'- -CGGGaaCUCCUGGCCCuCGAG-Ga--ACU- -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 101929 | 0.67 | 0.763754 |
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Target: 5'- cGCCCUgGAGacCCGGGGGC-CCUg-- -3' miRNA: 3'- -CGGGAaCUCcuGGCCCUCGaGGAacu -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 102388 | 0.69 | 0.695382 |
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Target: 5'- gGCCCUUGuGGACCcauGGGGC-CCg--- -3' miRNA: 3'- -CGGGAACuCCUGGc--CCUCGaGGaacu -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 51015 | 0.69 | 0.695382 |
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Target: 5'- -gCCUUGAGGGCCccGGGGCcCCUUa- -3' miRNA: 3'- cgGGAACUCCUGGc-CCUCGaGGAAcu -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 50842 | 0.7 | 0.634606 |
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Target: 5'- gGCCCUUGGGucCUGGcAGCcCCUUGGg -3' miRNA: 3'- -CGGGAACUCcuGGCCcUCGaGGAACU- -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 41844 | 0.7 | 0.624423 |
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Target: 5'- gGCCCgcGAGGAcccCCGGGGGCgCCg--- -3' miRNA: 3'- -CGGGaaCUCCU---GGCCCUCGaGGaacu -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 102131 | 0.71 | 0.563697 |
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Target: 5'- gGUCCUgGGGGGCCaGGAGgUCCUgggGGg -3' miRNA: 3'- -CGGGAaCUCCUGGcCCUCgAGGAa--CU- -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 101863 | 0.72 | 0.495054 |
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Target: 5'- cGCCCUuuggacccUGAGGcCCGGGAGggCCUa-- -3' miRNA: 3'- -CGGGA--------ACUCCuGGCCCUCgaGGAacu -5' |
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| 30631 | 5' | -57.5 | NC_006549.1 | + | 49872 | 0.75 | 0.339758 |
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Target: 5'- aCCCUUGGGGcCCGGGGGC-CCa--- -3' miRNA: 3'- cGGGAACUCCuGGCCCUCGaGGaacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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