Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30632 | 5' | -65.5 | NC_006549.1 | + | 49629 | 0.72 | 0.174486 |
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Target: 5'- cUUUCCAGGa-GGUCCUCuAGGCCCgGGu -3' miRNA: 3'- -AAAGGUCCccCCGGGAG-UCCGGGgCC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 41830 | 0.73 | 0.158435 |
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Target: 5'- --cCCGGGGgcgccGGGCCCgagaGGGCCCgCGGg -3' miRNA: 3'- aaaGGUCCC-----CCCGGGag--UCCGGG-GCC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 7718 | 0.74 | 0.147285 |
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Target: 5'- --cCCAGGGGGGCCgaUGGGCCgCaGGg -3' miRNA: 3'- aaaGGUCCCCCCGGgaGUCCGGgG-CC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 41724 | 0.75 | 0.124003 |
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Target: 5'- --cCCAGGGGcGCCCgCGGGCCCCc- -3' miRNA: 3'- aaaGGUCCCCcCGGGaGUCCGGGGcc -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 102130 | 0.75 | 0.118005 |
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Target: 5'- -gUCCuGGGGGGCCaggAGGUCCUGGg -3' miRNA: 3'- aaAGGuCCCCCCGGgagUCCGGGGCC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 50850 | 0.78 | 0.071303 |
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Target: 5'- --cCCAGGuGGGCCCUUGGGUCCUGGc -3' miRNA: 3'- aaaGGUCCcCCCGGGAGUCCGGGGCC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 101919 | 0.8 | 0.053793 |
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Target: 5'- --aCCc-GGGGGCCCUgCAGGCCCUGGg -3' miRNA: 3'- aaaGGucCCCCCGGGA-GUCCGGGGCC- -5' |
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| 30632 | 5' | -65.5 | NC_006549.1 | + | 49854 | 0.8 | 0.049792 |
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Target: 5'- --cCCAGGGGGGCCCggAGGCCCUu- -3' miRNA: 3'- aaaGGUCCCCCCGGGagUCCGGGGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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