Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30633 | 5' | -53 | NC_006549.1 | + | 36345 | 0.66 | 0.974642 |
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Target: 5'- gCGACgGCGcgaaacauUGGcaCGCGCGAaaaAUGCGAa -3' miRNA: 3'- -GCUGgUGC--------ACCuaGCGCGUU---UACGCU- -5' |
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| 30633 | 5' | -53 | NC_006549.1 | + | 96131 | 0.66 | 0.97302 |
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Target: 5'- gCGGCCGCgGUGGAacaaaaauuuccaccUCcucauuuuacaGUGCAAGUGCGc -3' miRNA: 3'- -GCUGGUG-CACCU---------------AG-----------CGCGUUUACGCu -5' |
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| 30633 | 5' | -53 | NC_006549.1 | + | 72755 | 0.66 | 0.970742 |
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Target: 5'- aCGGCCGCGUGGuuaaauaaacaGCuGCAAAUuCGAc -3' miRNA: 3'- -GCUGGUGCACCuag--------CG-CGUUUAcGCU- -5' |
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| 30633 | 5' | -53 | NC_006549.1 | + | 54164 | 0.68 | 0.925168 |
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Target: 5'- aCGACCGCGUGGAaacggcgguuaagcUCuuuGCGCGAGgucagGCc- -3' miRNA: 3'- -GCUGGUGCACCU--------------AG---CGCGUUUa----CGcu -5' |
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| 30633 | 5' | -53 | NC_006549.1 | + | 116763 | 0.69 | 0.8828 |
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Target: 5'- uGACCACGcGGGUUGCGCccacgggGCa- -3' miRNA: 3'- gCUGGUGCaCCUAGCGCGuuua---CGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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