Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30634 | 3' | -54 | NC_006549.1 | + | 85150 | 0.66 | 0.955158 |
Target: 5'- aAUGGuuGuGCUCUCCGUu-UCUuaaACCGCg -3' miRNA: 3'- -UGCUgcC-CGAGAGGCAuuAGA---UGGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 90770 | 0.67 | 0.942366 |
Target: 5'- gGCGAUGGaGCaauUCguaCCGUuGUCU-CCGCa -3' miRNA: 3'- -UGCUGCC-CG---AGa--GGCAuUAGAuGGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 116216 | 0.67 | 0.937624 |
Target: 5'- -gGGCGGGCaCguaGUAAUUUAUCGCg -3' miRNA: 3'- ugCUGCCCGaGaggCAUUAGAUGGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 134667 | 0.68 | 0.890958 |
Target: 5'- gACGGCGGGCauuUCgUCCGUuuUCUcgUGCg -3' miRNA: 3'- -UGCUGCCCG---AG-AGGCAuuAGAugGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 23779 | 0.68 | 0.884054 |
Target: 5'- -aGGCGGGCUCUauaCGUGcgCagaUAgCGCa -3' miRNA: 3'- ugCUGCCCGAGAg--GCAUuaG---AUgGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 51716 | 0.69 | 0.861993 |
Target: 5'- aACGcCGGGC---CCGUuugCUACCGCg -3' miRNA: 3'- -UGCuGCCCGagaGGCAuuaGAUGGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 60650 | 0.7 | 0.838032 |
Target: 5'- -aGAuCGGGCgguUCUCCcUuuUCUGCCGCc -3' miRNA: 3'- ugCU-GCCCG---AGAGGcAuuAGAUGGCG- -5' |
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30634 | 3' | -54 | NC_006549.1 | + | 105502 | 0.73 | 0.65608 |
Target: 5'- -aGAgGGG-UUUCCGUuucGUCUGCCGCg -3' miRNA: 3'- ugCUgCCCgAGAGGCAu--UAGAUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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