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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30634 | 5' | -53 | NC_006549.1 | + | 7653 | 0.66 | 0.974845 |
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Target: 5'- -gGCGGguauuccggGUCGC-AGAGGCGCCa--- -3' miRNA: 3'- ggCGCCa--------UAGUGcUCUCUGCGGauaa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 34888 | 0.67 | 0.951052 |
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Target: 5'- cUCGCGGg--CugGGGAGAcaaCGCCg--- -3' miRNA: 3'- -GGCGCCauaGugCUCUCU---GCGGauaa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 46946 | 0.68 | 0.931742 |
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Target: 5'- aCCGggcgaauCGGUAgccgaUCACGcGAGACGCCUc-- -3' miRNA: 3'- -GGC-------GCCAU-----AGUGCuCUCUGCGGAuaa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 96855 | 0.68 | 0.926943 |
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Target: 5'- aCGaCGGUA-CAgUGAGGGACGCCa--- -3' miRNA: 3'- gGC-GCCAUaGU-GCUCUCUGCGGauaa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 29280 | 0.69 | 0.882762 |
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Target: 5'- gCCGUGGc--CACGGGAGuCGCCg--- -3' miRNA: 3'- -GGCGCCauaGUGCUCUCuGCGGauaa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 78209 | 0.71 | 0.804212 |
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Target: 5'- uCCGagGGUGUCACGugcgcauaucucuuGAGGCGUCUGUa -3' miRNA: 3'- -GGCg-CCAUAGUGCu-------------CUCUGCGGAUAa -5' |
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| 30634 | 5' | -53 | NC_006549.1 | + | 7025 | 0.76 | 0.536429 |
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Target: 5'- cCCGCGGggGUUAgGGGAGAUGCCg--- -3' miRNA: 3'- -GGCGCCa-UAGUgCUCUCUGCGGauaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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