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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30636 | 3' | -49.2 | NC_006549.1 | + | 126132 | 0.66 | 0.997731 |
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Target: 5'- -uGCAGAUUGuuGACAUUGCCGAc-- -3' miRNA: 3'- cuUGUCUGGCugUUGUAGCGGUUaga -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 35568 | 0.66 | 0.996786 |
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Target: 5'- aAAUGGGCCGACAAUuggcUCGuguCCGAUCa -3' miRNA: 3'- cUUGUCUGGCUGUUGu---AGC---GGUUAGa -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 82807 | 0.67 | 0.99186 |
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Target: 5'- gGAGCGGGCuUGACGGCGUcCGCCu---- -3' miRNA: 3'- -CUUGUCUG-GCUGUUGUA-GCGGuuaga -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 70336 | 0.68 | 0.986108 |
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Target: 5'- --uUAGAUaggGACGACAUCGCCAAc-- -3' miRNA: 3'- cuuGUCUGg--CUGUUGUAGCGGUUaga -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 126411 | 0.7 | 0.954655 |
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Target: 5'- aAGCAGACUGGCGGCA-CGCacaacaaGAUCUu -3' miRNA: 3'- cUUGUCUGGCUGUUGUaGCGg------UUAGA- -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 84016 | 0.73 | 0.893297 |
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Target: 5'- cGAACuGAUUGACAACAUCGCgCAGc-- -3' miRNA: 3'- -CUUGuCUGGCUGUUGUAGCG-GUUaga -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 77587 | 0.76 | 0.742464 |
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Target: 5'- gGGAUGGGCCGAuCAGCAUgGCCGcgCUa -3' miRNA: 3'- -CUUGUCUGGCU-GUUGUAgCGGUuaGA- -5' |
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| 30636 | 3' | -49.2 | NC_006549.1 | + | 39782 | 0.76 | 0.732126 |
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Target: 5'- cAACAGAUUGGCGAUGUUGUCGGUCUg -3' miRNA: 3'- cUUGUCUGGCUGUUGUAGCGGUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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