miRNA: 3'- -CCAgaaUUGUUu--GCGGGGGCGUCGau -5'
miRNA: 3'- cCAGAAUUGUUUGCGGgGGCgUCgau -5'
miRNA: 3'- cCAGAa---UUGU-UUGCGGGGGCG---UCGau -5'
miRNA: 3'- -CCAGAAUUGU--UUGCgGGGGCGUcgau -5'
miRNA: 3'- -CCAGAa---UUGUUUGCGGGgGCG---------UCGAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30642 | 3' | -52.8 | NC_006549.1 | + | 52946 | 0.66 | 0.969785 |
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Target: 5'- cGGUa--AACGGcgcCGCCCUCGCGGCc- -3' miRNA: 3'- -CCAgaaUUGUUu--GCGGGGGCGUCGau -5' |
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| 30642 | 3' | -52.8 | NC_006549.1 | + | 72459 | 0.66 | 0.959611 |
|
Target: 5'- aGUUUUGACAAACGCCgCUGgGGg-- -3' miRNA: 3'- cCAGAAUUGUUUGCGGgGGCgUCgau -5' |
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| 30642 | 3' | -52.8 | NC_006549.1 | + | 35184 | 0.67 | 0.947295 |
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Target: 5'- uGUCUcaggAACAgGGCGCCUCCGCcuuGGCg- -3' miRNA: 3'- cCAGAa---UUGU-UUGCGGGGGCG---UCGau -5' |
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| 30642 | 3' | -52.8 | NC_006549.1 | + | 133717 | 0.68 | 0.903318 |
|
Target: 5'- aGGUCUUAACGgaGACG-CCCCGUAa--- -3' miRNA: 3'- -CCAGAAUUGU--UUGCgGGGGCGUcgau -5' |
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| 30642 | 3' | -52.8 | NC_006549.1 | + | 104412 | 0.69 | 0.867663 |
|
Target: 5'- gGGUCUaucgGGCGAAUGCCCgUGCuuuuugagaAGCUGa -3' miRNA: 3'- -CCAGAa---UUGUUUGCGGGgGCG---------UCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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