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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30645 | 3' | -45.1 | NC_006549.1 | + | 16262 | 0.66 | 0.99999 |
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Target: 5'- gAGGGCuagGAGGCGUCGGAGggGUGGg- -3' miRNA: 3'- -UCUUGu--UUCUGUGGCCUUuaCACUaa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 51462 | 0.68 | 0.999834 |
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Target: 5'- gGGAACAGGGAUugGCCGuGGAAUGg---- -3' miRNA: 3'- -UCUUGUUUCUG--UGGC-CUUUACacuaa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 101146 | 0.7 | 0.99835 |
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Target: 5'- aAGGuUAAAGAUGuuGGGAAUGUGAa- -3' miRNA: 3'- -UCUuGUUUCUGUggCCUUUACACUaa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 118109 | 0.7 | 0.99835 |
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Target: 5'- -aAugGAAGACACUGGAAcguguuUGUGGUg -3' miRNA: 3'- ucUugUUUCUGUGGCCUUu-----ACACUAa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 16181 | 0.7 | 0.997998 |
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Target: 5'- gGGGAguaGGAGGCGUCGGAGGUGUGGg- -3' miRNA: 3'- -UCUUg--UUUCUGUGGCCUUUACACUaa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 95611 | 0.72 | 0.993359 |
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Target: 5'- cGGAauuguGCAAAGGCGCUGGAGccaggGUGGUg -3' miRNA: 3'- -UCU-----UGUUUCUGUGGCCUUua---CACUAa -5' |
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| 30645 | 3' | -45.1 | NC_006549.1 | + | 42440 | 0.73 | 0.980253 |
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Target: 5'- aGGAugAAAGGCAUgGGAAAaGUGGUc -3' miRNA: 3'- -UCUugUUUCUGUGgCCUUUaCACUAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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