Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30645 | 5' | -59 | NC_006549.1 | + | 56901 | 0.66 | 0.790259 |
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Target: 5'- -aACCGGC--GGCCGcuGCGGGCagggUGUCa -3' miRNA: 3'- gaUGGCUGuaUCGGC--CGCCCGa---GCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 50434 | 0.66 | 0.781261 |
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Target: 5'- -gACCGGCGgGGCCcauuGGUccGGGCuUCGUCu -3' miRNA: 3'- gaUGGCUGUaUCGG----CCG--CCCG-AGCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 6179 | 0.66 | 0.75353 |
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Target: 5'- gUGCCGcACAcc-CCGGCGGcGCUgGCg -3' miRNA: 3'- gAUGGC-UGUaucGGCCGCC-CGAgCGg -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 74545 | 0.67 | 0.74407 |
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Target: 5'- -aACUGACGgaaauaggGGCCGuGUGGGCggaucCGUCa -3' miRNA: 3'- gaUGGCUGUa-------UCGGC-CGCCCGa----GCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 5442 | 0.68 | 0.645711 |
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Target: 5'- uCUAUgGGCA-GGgUGGCGGGCgCGCUa -3' miRNA: 3'- -GAUGgCUGUaUCgGCCGCCCGaGCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 83575 | 0.68 | 0.645711 |
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Target: 5'- -cACCGGUAUGGUgGGgGGGggCGCCc -3' miRNA: 3'- gaUGGCUGUAUCGgCCgCCCgaGCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 34027 | 0.69 | 0.615631 |
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Target: 5'- --cCCGACGa---CGGCGGGUgguUCGCCa -3' miRNA: 3'- gauGGCUGUaucgGCCGCCCG---AGCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 135098 | 0.71 | 0.517123 |
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Target: 5'- -cGCCGACA----UGGUGGaGCUCGCCu -3' miRNA: 3'- gaUGGCUGUaucgGCCGCC-CGAGCGG- -5' |
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| 30645 | 5' | -59 | NC_006549.1 | + | 82820 | 0.71 | 0.488711 |
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Target: 5'- -aGCCGGCGcuGCCGgaGCGGGCUugacggcgucCGCCu -3' miRNA: 3'- gaUGGCUGUauCGGC--CGCCCGA----------GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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