Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30648 | 5' | -54 | NC_006549.1 | + | 18774 | 0.66 | 0.96998 |
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Target: 5'- uGUUGaaagaaaauucUGCGCGUCCGCuGUuguCgGACGc -3' miRNA: 3'- gCAAC-----------AUGCGCAGGCG-CAu--GgCUGCu -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 68867 | 0.66 | 0.963712 |
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Target: 5'- ---cGUGCGCGUgcgauaccCUGUGgaUACCGGCGGg -3' miRNA: 3'- gcaaCAUGCGCA--------GGCGC--AUGGCUGCU- -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 97385 | 0.66 | 0.963712 |
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Target: 5'- ---gGUACGUagauuGUCUGCcgGCCGACGAc -3' miRNA: 3'- gcaaCAUGCG-----CAGGCGcaUGGCUGCU- -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 82842 | 0.66 | 0.960258 |
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Target: 5'- uCGUgggGUGgGUaUCCGCGggaGCCGGCGc -3' miRNA: 3'- -GCAa--CAUgCGcAGGCGCa--UGGCUGCu -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 73724 | 0.69 | 0.887576 |
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Target: 5'- uCGUcgGUACGCGgaCGCGUACaaCGACGu -3' miRNA: 3'- -GCAa-CAUGCGCagGCGCAUG--GCUGCu -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 139785 | 0.7 | 0.834535 |
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Target: 5'- uGUcuUGCGCGUCUGCcgacGCCGugGAa -3' miRNA: 3'- gCAacAUGCGCAGGCGca--UGGCugCU- -5' |
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| 30648 | 5' | -54 | NC_006549.1 | + | 64010 | 0.72 | 0.743945 |
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Target: 5'- cCGUggcGUACGCGgugcCCGCGUucuCCGGCa- -3' miRNA: 3'- -GCAa--CAUGCGCa---GGCGCAu--GGCUGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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