Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30649 | 3' | -62.2 | NC_006549.1 | + | 48139 | 0.66 | 0.60581 |
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Target: 5'- gGACAUGGCACUgCCCgaaagcUGgGGCCgGCc -3' miRNA: 3'- gUUGUGCCGUGG-GGG------ACgCCGGaCGc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 21268 | 0.68 | 0.462719 |
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Target: 5'- cCAGCGCGGCGCCCaauauaggcUCggGCGGCauggGCa -3' miRNA: 3'- -GUUGUGCCGUGGG---------GGa-CGCCGga--CGc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 98988 | 0.68 | 0.453765 |
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Target: 5'- gAGCGCGGCACCCaagGCG-CCcGCGc -3' miRNA: 3'- gUUGUGCCGUGGGggaCGCcGGaCGC- -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 83776 | 0.69 | 0.427485 |
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Target: 5'- aAGCACGuGCGCCCUCaacGCGGCCa--- -3' miRNA: 3'- gUUGUGC-CGUGGGGGa--CGCCGGacgc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 52941 | 0.7 | 0.385761 |
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Target: 5'- ---aACGGCGCCgCCCUcGCGGCCg--- -3' miRNA: 3'- guugUGCCGUGG-GGGA-CGCCGGacgc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 41702 | 0.7 | 0.377745 |
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Target: 5'- cCGGCcccccCGGC-CCCCCUG-GGCCcGCGg -3' miRNA: 3'- -GUUGu----GCCGuGGGGGACgCCGGaCGC- -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 57362 | 0.72 | 0.290546 |
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Target: 5'- gCAACACGGCgu-CCCUGCGaGCCUGa- -3' miRNA: 3'- -GUUGUGCCGuggGGGACGC-CGGACgc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 60998 | 0.73 | 0.25322 |
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Target: 5'- uGACACagauGGCGCUgCCgcacgGCGGCCUGCc -3' miRNA: 3'- gUUGUG----CCGUGGgGGa----CGCCGGACGc -5' |
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| 30649 | 3' | -62.2 | NC_006549.1 | + | 52330 | 0.77 | 0.125046 |
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Target: 5'- aGACACGGCACCCgUCUauuugGCGGCgUGCGc -3' miRNA: 3'- gUUGUGCCGUGGG-GGA-----CGCCGgACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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