Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30649 | 5' | -50.8 | NC_006549.1 | + | 23432 | 0.66 | 0.988009 |
Target: 5'- aAGCGCAAACGUgACgUGuuuccuaggugGCGCAuGCGUu -3' miRNA: 3'- -UCGCGUUUGCAgUGgAC-----------UGUGU-UGUA- -5' |
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30649 | 5' | -50.8 | NC_006549.1 | + | 3666 | 0.66 | 0.988009 |
Target: 5'- uAGCGCAAACGUUg---GAC-CAACAg -3' miRNA: 3'- -UCGCGUUUGCAGuggaCUGuGUUGUa -5' |
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30649 | 5' | -50.8 | NC_006549.1 | + | 57026 | 0.66 | 0.988009 |
Target: 5'- --gGgAAACGUCgcGCCUGACGCcACAa -3' miRNA: 3'- ucgCgUUUGCAG--UGGACUGUGuUGUa -5' |
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30649 | 5' | -50.8 | NC_006549.1 | + | 120560 | 0.66 | 0.982577 |
Target: 5'- aGGCGCGAGUGUCggACC--GCGCAACAc -3' miRNA: 3'- -UCGCGUUUGCAG--UGGacUGUGUUGUa -5' |
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30649 | 5' | -50.8 | NC_006549.1 | + | 98110 | 0.68 | 0.962978 |
Target: 5'- cGUGCGGugccACGUCACCgGACGCGu--- -3' miRNA: 3'- uCGCGUU----UGCAGUGGaCUGUGUugua -5' |
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30649 | 5' | -50.8 | NC_006549.1 | + | 4383 | 0.68 | 0.962978 |
Target: 5'- uGCGagagauaAAGCGUCACCUGAUucccaGACAc -3' miRNA: 3'- uCGCg------UUUGCAGUGGACUGug---UUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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