miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30650 5' -55.6 NC_006549.1 + 97878 0.66 0.908975
Target:  5'- gACGGCGACGa-AGGuaaccuguauuuGCCgcaagguccccaGGCCAUCg -3'
miRNA:   3'- gUGCCGCUGCaaUCCu-----------UGG------------CCGGUAG- -5'
30650 5' -55.6 NC_006549.1 + 52761 0.67 0.886863
Target:  5'- aCGCGGCaACGUUccccgucgcaggAGGAACaagcggcggagcgcgCGGCCAUg -3'
miRNA:   3'- -GUGCCGcUGCAA------------UCCUUG---------------GCCGGUAg -5'
30650 5' -55.6 NC_006549.1 + 128781 0.67 0.884085
Target:  5'- gACaGCGGCGUaUAcGAACCuGCCGUCu -3'
miRNA:   3'- gUGcCGCUGCA-AUcCUUGGcCGGUAG- -5'
30650 5' -55.6 NC_006549.1 + 38595 0.67 0.849632
Target:  5'- uGCGGCGACGUcgUAauuuaacgccccuauGGAACCGuCCAa- -3'
miRNA:   3'- gUGCCGCUGCA--AU---------------CCUUGGCcGGUag -5'
30650 5' -55.6 NC_006549.1 + 48563 0.71 0.66748
Target:  5'- aGCGGCGACaGUcAGGAACC-GUUGUCg -3'
miRNA:   3'- gUGCCGCUG-CAaUCCUUGGcCGGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.