Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30651 | 5' | -55.5 | NC_006549.1 | + | 59960 | 0.66 | 0.9009 |
Target: 5'- cCGgGUGGGAAAGGgaCAUAUaggCGGCg -3' miRNA: 3'- aGCgCGCUCUUUCCg-GUAUAg--GCCGa -5' |
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30651 | 5' | -55.5 | NC_006549.1 | + | 21228 | 0.67 | 0.858051 |
Target: 5'- -gGCGgcCGAGGuguuguccgGAGGCCAUcugCCGGCg -3' miRNA: 3'- agCGC--GCUCU---------UUCCGGUAua-GGCCGa -5' |
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30651 | 5' | -55.5 | NC_006549.1 | + | 103220 | 0.68 | 0.807722 |
Target: 5'- uUCGCGCGuGAGAGGCaCAUGcgCUcGCc -3' miRNA: 3'- -AGCGCGCuCUUUCCG-GUAUa-GGcCGa -5' |
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30651 | 5' | -55.5 | NC_006549.1 | + | 54135 | 0.71 | 0.660671 |
Target: 5'- uUUGCGCGAGGucAGGCCcgcgcCCGGUa -3' miRNA: 3'- -AGCGCGCUCUu-UCCGGuaua-GGCCGa -5' |
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30651 | 5' | -55.5 | NC_006549.1 | + | 1673 | 0.74 | 0.488113 |
Target: 5'- cCGCGUGGGaAGAGGCCAUcgCCGaCa -3' miRNA: 3'- aGCGCGCUC-UUUCCGGUAuaGGCcGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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