Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30657 | 3' | -44.8 | NC_006549.1 | + | 34650 | 0.66 | 0.999984 |
Target: 5'- -cGGCUugGGAauGGCGCGG-AGAUGUUUCu -3' miRNA: 3'- uuUCGA--UCU--CUGCGUCuUUUAUAGAG- -5' |
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30657 | 3' | -44.8 | NC_006549.1 | + | 3590 | 0.7 | 0.998222 |
Target: 5'- --cGCUAGAGGCGCGGGc-----CUCg -3' miRNA: 3'- uuuCGAUCUCUGCGUCUuuuauaGAG- -5' |
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30657 | 3' | -44.8 | NC_006549.1 | + | 119628 | 0.72 | 0.992163 |
Target: 5'- gGGAGCUGGGGGCGgAGggGcgugcuguaugccugGUAUuCUCa -3' miRNA: 3'- -UUUCGAUCUCUGCgUCuuU---------------UAUA-GAG- -5' |
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30657 | 3' | -44.8 | NC_006549.1 | + | 104574 | 0.73 | 0.981226 |
Target: 5'- -uGGCUGuuGACG-AGAAAAUAUCUCg -3' miRNA: 3'- uuUCGAUcuCUGCgUCUUUUAUAGAG- -5' |
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30657 | 3' | -44.8 | NC_006549.1 | + | 9487 | 0.75 | 0.963111 |
Target: 5'- gAGAGCUuuggacGAGugGcCAGAuAAUGUCUCg -3' miRNA: 3'- -UUUCGAu-----CUCugC-GUCUuUUAUAGAG- -5' |
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30657 | 3' | -44.8 | NC_006549.1 | + | 26671 | 0.75 | 0.955006 |
Target: 5'- --uGCUAuAGACGCGGAgggcggucuAAAUGUCUCg -3' miRNA: 3'- uuuCGAUcUCUGCGUCU---------UUUAUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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