Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30658 | 5' | -56.3 | NC_006549.1 | + | 6138 | 0.67 | 0.820755 |
Target: 5'- -aGGGUAcaagacUGGcGACCUgucugGUacggGCACGCCg -3' miRNA: 3'- aaCCCAU------ACC-CUGGAa----CGg---UGUGCGG- -5' |
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30658 | 5' | -56.3 | NC_006549.1 | + | 50974 | 0.68 | 0.736437 |
Target: 5'- -aGGGgcccAUGGGGCCcuucggGCCuuGCACGUCc -3' miRNA: 3'- aaCCCa---UACCCUGGaa----CGG--UGUGCGG- -5' |
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30658 | 5' | -56.3 | NC_006549.1 | + | 5950 | 0.69 | 0.716428 |
Target: 5'- -cGGGcGUGGGGCCUUugCAgGCGUCa -3' miRNA: 3'- aaCCCaUACCCUGGAAcgGUgUGCGG- -5' |
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30658 | 5' | -56.3 | NC_006549.1 | + | 50057 | 0.71 | 0.613425 |
Target: 5'- gUGGGUccuacGGGACCUaUGUCucccucCACGCCg -3' miRNA: 3'- aACCCAua---CCCUGGA-ACGGu-----GUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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