Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30659 | 5' | -57.5 | NC_006549.1 | + | 113449 | 0.66 | 0.866642 |
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Target: 5'- gCCUACGUAGUcGGAcgaauCCUgAUCUgCGGGu -3' miRNA: 3'- -GGGUGCAUCA-CCUc----GGA-UAGGgGCCC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 7026 | 0.66 | 0.843813 |
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Target: 5'- gCCCGCGgGGguuaggGGAgauGCCggcgCUCCGGGa -3' miRNA: 3'- -GGGUGCaUCa-----CCU---CGGaua-GGGGCCC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 101928 | 0.67 | 0.835827 |
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Target: 5'- gCCCugG-AGacccgGGGGCCcugcaggCCCUGGGa -3' miRNA: 3'- -GGGugCaUCa----CCUCGGaua----GGGGCCC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 49727 | 0.67 | 0.81933 |
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Target: 5'- cCCCGCGggcccGUGGGGCCcguuggCCCgCGaGGu -3' miRNA: 3'- -GGGUGCau---CACCUCGGaua---GGG-GC-CC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 46330 | 0.67 | 0.814251 |
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Target: 5'- gCCACGUggaaagaguccgAGUGGuGCCUuuugaagccaccuuuGUCaCuCCGGGg -3' miRNA: 3'- gGGUGCA------------UCACCuCGGA---------------UAG-G-GGCCC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 97066 | 0.67 | 0.810833 |
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Target: 5'- cCUCGCGU-GUGGAGCCaagAUCagaUCGGa -3' miRNA: 3'- -GGGUGCAuCACCUCGGa--UAGg--GGCCc -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 119920 | 0.71 | 0.619395 |
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Target: 5'- uCUUugGcGGUGGAGCUcccccGUCUCCGGGu -3' miRNA: 3'- -GGGugCaUCACCUCGGa----UAGGGGCCC- -5' |
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| 30659 | 5' | -57.5 | NC_006549.1 | + | 116595 | 0.73 | 0.473826 |
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Target: 5'- uUCCACaGUGGgucuGAGCCUAUCUuuGGGc -3' miRNA: 3'- -GGGUG-CAUCac--CUCGGAUAGGggCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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