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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30666 | 3' | -57.2 | NC_006549.1 | + | 77835 | 0.66 | 0.849165 |
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Target: 5'- --aGCCUCUGUcuuGGCgGcgGCuGGCg -3' miRNA: 3'- cuaCGGAGGCGuuuCCGgCuaCG-CCG- -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 10815 | 0.66 | 0.841096 |
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Target: 5'- --aGCCgCCGCAAAuGUCGcUGCGGa -3' miRNA: 3'- cuaCGGaGGCGUUUcCGGCuACGCCg -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 122444 | 0.66 | 0.832835 |
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Target: 5'- -cUGUUUgCCGCAucGGCCuuUGCGGa -3' miRNA: 3'- cuACGGA-GGCGUuuCCGGcuACGCCg -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 7955 | 0.67 | 0.788011 |
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Target: 5'- --gGUCUCCGCGGAGcGCCcggacccGAUGgGGa -3' miRNA: 3'- cuaCGGAGGCGUUUC-CGG-------CUACgCCg -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 12638 | 0.67 | 0.788011 |
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Target: 5'- --cGUCUCCuaCAGAGGCCGuauauucGUGCGuGCc -3' miRNA: 3'- cuaCGGAGGc-GUUUCCGGC-------UACGC-CG- -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 122472 | 0.67 | 0.77031 |
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Target: 5'- --gGCUUuuGCAAAGGCCucuuCGGCu -3' miRNA: 3'- cuaCGGAggCGUUUCCGGcuacGCCG- -5' |
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| 30666 | 3' | -57.2 | NC_006549.1 | + | 35168 | 0.69 | 0.69161 |
|
Target: 5'- --cGCCUCCGCcuuGGCgcaGGUGCuGCu -3' miRNA: 3'- cuaCGGAGGCGuuuCCGg--CUACGcCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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