Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30667 | 3' | -53.7 | NC_006549.1 | + | 120837 | 0.66 | 0.954936 |
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Target: 5'- aGAGGCUGccguAAGAuGGCUUCCUaaaGAAuuGGa -3' miRNA: 3'- cUUCCGGC----UUCU-CCGAAGGAg--CUU--CC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 75038 | 0.66 | 0.950856 |
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Target: 5'- -cAGGCCGcAGGGGGUgccgUgUUGggGGa -3' miRNA: 3'- cuUCCGGC-UUCUCCGaa--GgAGCuuCC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 49860 | 0.66 | 0.946536 |
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Target: 5'- cGggGGCCcAGGGGGg--CC-CGGAGGc -3' miRNA: 3'- -CuuCCGGcUUCUCCgaaGGaGCUUCC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 16147 | 0.67 | 0.937166 |
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Target: 5'- aGGAGGCUuGGGGGGggUCUggGGAGGg -3' miRNA: 3'- -CUUCCGGcUUCUCCgaAGGagCUUCC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 10265 | 0.67 | 0.921256 |
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Target: 5'- --uGGCCGAgcuGGAGGCgaacgCCUUGAc-- -3' miRNA: 3'- cuuCCGGCU---UCUCCGaa---GGAGCUucc -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 16263 | 0.68 | 0.8828 |
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Target: 5'- gGAGGGCU-AGGAGGCg---UCGGAGGg -3' miRNA: 3'- -CUUCCGGcUUCUCCGaaggAGCUUCC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 117660 | 0.7 | 0.836926 |
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Target: 5'- uGAGGUCGAGGAGGCggcuuccaccagcucUCCUCGugcugcGGc -3' miRNA: 3'- cUUCCGGCUUCUCCGa--------------AGGAGCuu----CC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 102125 | 0.7 | 0.836087 |
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Target: 5'- gGggGGCC-AGGAGGU--CCUgGggGGc -3' miRNA: 3'- -CuuCCGGcUUCUCCGaaGGAgCuuCC- -5' |
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| 30667 | 3' | -53.7 | NC_006549.1 | + | 136720 | 0.75 | 0.558418 |
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Target: 5'- -cGGGCU--GGAGGacgUCCUCGAAGGg -3' miRNA: 3'- cuUCCGGcuUCUCCga-AGGAGCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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