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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30669 | 3' | -57.9 | NC_006549.1 | + | 52897 | 0.66 | 0.79803 |
Target: 5'- ----aGGUuugcaGGCgGAACCccuGCCCGAAGg -3' miRNA: 3'- uuugaCCG-----CCGgCUUGGu--CGGGCUUC- -5' |
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30669 | 3' | -57.9 | NC_006549.1 | + | 131443 | 0.66 | 0.785249 |
Target: 5'- ---aUGGCGGCCGucuuccaccauuCCGGCCacaGggGc -3' miRNA: 3'- uuugACCGCCGGCuu----------GGUCGGg--CuuC- -5' |
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30669 | 3' | -57.9 | NC_006549.1 | + | 50448 | 0.66 | 0.770311 |
Target: 5'- -cACUGGCcccGCCGGACCggcggGGCCCa--- -3' miRNA: 3'- uuUGACCGc--CGGCUUGG-----UCGGGcuuc -5' |
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30669 | 3' | -57.9 | NC_006549.1 | + | 21235 | 0.71 | 0.499354 |
Target: 5'- uGGGCagGGCGGCCGAGgUguuGUCCGGAGg -3' miRNA: 3'- -UUUGa-CCGCCGGCUUgGu--CGGGCUUC- -5' |
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30669 | 3' | -57.9 | NC_006549.1 | + | 83216 | 0.72 | 0.474487 |
Target: 5'- cAGACUGGCGuaCGGuACCguggcgcucacccagGGCCCGGAGa -3' miRNA: 3'- -UUUGACCGCcgGCU-UGG---------------UCGGGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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