Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30670 | 5' | -59.9 | NC_006549.1 | + | 82807 | 0.67 | 0.609238 |
Target: 5'- gGAGCGgGCUUg-ACggCGUCCGCCUa -3' miRNA: 3'- -CUCGCgCGAAagUGagGCGGGCGGAg -5' |
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30670 | 5' | -59.9 | NC_006549.1 | + | 102215 | 0.68 | 0.59914 |
Target: 5'- cGGCGCGCU---ACgaUGCCUGCCUCu -3' miRNA: 3'- cUCGCGCGAaagUGagGCGGGCGGAG- -5' |
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30670 | 5' | -59.9 | NC_006549.1 | + | 128659 | 0.68 | 0.589065 |
Target: 5'- uGGGCGCGUcUUguUUCCccugGCCCGCCa- -3' miRNA: 3'- -CUCGCGCGaAAguGAGG----CGGGCGGag -5' |
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30670 | 5' | -59.9 | NC_006549.1 | + | 64159 | 0.68 | 0.589065 |
Target: 5'- aGAGCGgGCgaUCcCUCCGCCCaGCa-- -3' miRNA: 3'- -CUCGCgCGaaAGuGAGGCGGG-CGgag -5' |
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30670 | 5' | -59.9 | NC_006549.1 | + | 103218 | 0.69 | 0.519748 |
Target: 5'- -cGCGCGUgagaggCACaUgCGCUCGCCUCu -3' miRNA: 3'- cuCGCGCGaaa---GUG-AgGCGGGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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