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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30675 | 3' | -48.4 | NC_006551.1 | + | 2061 | 0.67 | 0.569904 |
Target: 5'- cAUCcgaAGCCAACGCgaaaGUGUUGGUu -3' miRNA: 3'- -UAGag-UUGGUUGUGaaagCGCAACCG- -5' |
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30675 | 3' | -48.4 | NC_006551.1 | + | 2657 | 0.71 | 0.338269 |
Target: 5'- -cCUCAAUCcACAuauucCUUUCGCGUgGGCu -3' miRNA: 3'- uaGAGUUGGuUGU-----GAAAGCGCAaCCG- -5' |
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30675 | 3' | -48.4 | NC_006551.1 | + | 2105 | 0.98 | 0.00382 |
Target: 5'- cAUCUCAACCAACACUUUCGC-UUGGCu -3' miRNA: 3'- -UAGAGUUGGUUGUGAAAGCGcAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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