Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30684 | 3' | -53.9 | NC_006552.1 | + | 1087 | 1.11 | 0.001308 |
Target: 5'- aACAUUCCGGACGACCGCAAGCUGUGCc -3' miRNA: 3'- -UGUAAGGCCUGCUGGCGUUCGACACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 1967 | 0.72 | 0.490275 |
Target: 5'- ---cUCgCGGcACGugCGCcGGCUGUGCc -3' miRNA: 3'- uguaAG-GCC-UGCugGCGuUCGACACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 707 | 0.7 | 0.6188 |
Target: 5'- cGCAcgCUGG-CGGCCugGCAGGCgggGUGCu -3' miRNA: 3'- -UGUaaGGCCuGCUGG--CGUUCGa--CACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 26245 | 0.69 | 0.673655 |
Target: 5'- uGCgg-CCGGACGccAgCGCGAuGUUGUGCg -3' miRNA: 3'- -UGuaaGGCCUGC--UgGCGUU-CGACACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 22967 | 0.68 | 0.706181 |
Target: 5'- gUAUUCCuGGACGaacGCCGCuuGUUGUGa -3' miRNA: 3'- uGUAAGG-CCUGC---UGGCGuuCGACACg -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 26740 | 0.67 | 0.768975 |
Target: 5'- -uGUUCCuGGAUG-CCGCugguGGCcGUGCc -3' miRNA: 3'- ugUAAGG-CCUGCuGGCGu---UCGaCACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 2828 | 0.67 | 0.779015 |
Target: 5'- aGCAcUCCaGugGACCGCAA-CUGUuCg -3' miRNA: 3'- -UGUaAGGcCugCUGGCGUUcGACAcG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 54144 | 0.67 | 0.787922 |
Target: 5'- gGCGgccgCCGGGCuucagcaGGCUGUAGGC-GUGCu -3' miRNA: 3'- -UGUaa--GGCCUG-------CUGGCGUUCGaCACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 49562 | 0.66 | 0.798629 |
Target: 5'- gGCGUUCUGGugGcGCUGCucauccuGCUGguccucgGCg -3' miRNA: 3'- -UGUAAGGCCugC-UGGCGuu-----CGACa------CG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 30378 | 0.66 | 0.826723 |
Target: 5'- ---cUUCGGACGGCCG-AGGCg--GCg -3' miRNA: 3'- uguaAGGCCUGCUGGCgUUCGacaCG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 49863 | 0.66 | 0.834805 |
Target: 5'- ---cUCCGcGACGcacggccugcagcGCCGCAGGCUGcucgaGCu -3' miRNA: 3'- uguaAGGC-CUGC-------------UGGCGUUCGACa----CG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 49610 | 0.66 | 0.835693 |
Target: 5'- ---gUCCaGGGCGaaGCCGCGAGCacggGCg -3' miRNA: 3'- uguaAGG-CCUGC--UGGCGUUCGaca-CG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 22360 | 0.66 | 0.835693 |
Target: 5'- cCAgggUCUGGcgaguaACGAuCUGCugcuGGCUGUGCa -3' miRNA: 3'- uGUa--AGGCC------UGCU-GGCGu---UCGACACG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 33717 | 0.66 | 0.84445 |
Target: 5'- gACAgguaCUGGugGAUCGCAGuCUGcgGCg -3' miRNA: 3'- -UGUaa--GGCCugCUGGCGUUcGACa-CG- -5' |
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30684 | 3' | -53.9 | NC_006552.1 | + | 24920 | 0.66 | 0.84445 |
Target: 5'- cGCAUUUCcuuGACGuaguGCCGCAGGUcuucguugUGUGCc -3' miRNA: 3'- -UGUAAGGc--CUGC----UGGCGUUCG--------ACACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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