Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30688 | 5' | -59.2 | NC_006552.1 | + | 23674 | 0.71 | 0.290247 |
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Target: 5'- gGGCUGC-AUGGUGcugcuACCaCUCGcCGGCAu -3' miRNA: 3'- -CCGGCGuUGCCACu----UGG-GAGC-GCCGU- -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 17299 | 0.73 | 0.21565 |
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Target: 5'- cGGCCGCGugGCGGUGAguACCUggUCGUcgaagugaacGGCAa -3' miRNA: 3'- -CCGGCGU--UGCCACU--UGGG--AGCG----------CCGU- -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 25833 | 0.73 | 0.214014 |
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Target: 5'- aGGCCgGCAGCGGUacggaaacuugaacGggUCCgacUCGCGGCu -3' miRNA: 3'- -CCGG-CGUUGCCA--------------CuuGGG---AGCGCCGu -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 29555 | 0.74 | 0.194701 |
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Target: 5'- gGGCCucguagaucGCAuucaGGUGGGCgCUCGCGGCGc -3' miRNA: 3'- -CCGG---------CGUug--CCACUUGgGAGCGCCGU- -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 45778 | 0.74 | 0.184909 |
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Target: 5'- aGGCCGCAuCGGgacGAACCCUacggGgGGCGu -3' miRNA: 3'- -CCGGCGUuGCCa--CUUGGGAg---CgCCGU- -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 53448 | 0.77 | 0.116977 |
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Target: 5'- aGGCCGgAGCGGcGAauggcacgcucggcAUCCUCGCGGCu -3' miRNA: 3'- -CCGGCgUUGCCaCU--------------UGGGAGCGCCGu -5' |
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| 30688 | 5' | -59.2 | NC_006552.1 | + | 1985 | 1.09 | 0.000506 |
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Target: 5'- uGGCCGCAACGGUGAACCCUCGCGGCAc -3' miRNA: 3'- -CCGGCGUUGCCACUUGGGAGCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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