Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30689 | 5' | -54.7 | NC_006552.1 | + | 2073 | 1.09 | 0.001403 |
Target: 5'- uUGAUCCUCAUCGAGCGCGGCGAACACc -3' miRNA: 3'- -ACUAGGAGUAGCUCGCGCCGCUUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 12807 | 0.76 | 0.264628 |
Target: 5'- cGcgCCUCAUUGGGCGCaGGCaguggucacuaccGAGCACg -3' miRNA: 3'- aCuaGGAGUAGCUCGCG-CCG-------------CUUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 40146 | 0.74 | 0.339024 |
Target: 5'- -cGUCUUCcgCGAaacgaccguagggGCGCGGCGAAUACa -3' miRNA: 3'- acUAGGAGuaGCU-------------CGCGCCGCUUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 21660 | 0.73 | 0.377137 |
Target: 5'- --cUCCUCGUCucGCGCGGCGucccgaacagcuuucGACGCg -3' miRNA: 3'- acuAGGAGUAGcuCGCGCCGC---------------UUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 16645 | 0.71 | 0.47754 |
Target: 5'- cUGAUCCUCAcucgcCGAGU-CGGCGAaacccuGCACa -3' miRNA: 3'- -ACUAGGAGUa----GCUCGcGCCGCU------UGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 8389 | 0.7 | 0.572078 |
Target: 5'- gGAUCCgc-UCGAGCGCgcccGGCGGAUg- -3' miRNA: 3'- aCUAGGaguAGCUCGCG----CCGCUUGug -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 53769 | 0.67 | 0.713561 |
Target: 5'- uUGAU-CUUAUCcGGCuCGGCGGGCGCc -3' miRNA: 3'- -ACUAgGAGUAGcUCGcGCCGCUUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 880 | 0.66 | 0.759567 |
Target: 5'- cUGGUCCUCAUCaaGAGCGaCgaccaggaagaugacGGUGAGC-Cg -3' miRNA: 3'- -ACUAGGAGUAG--CUCGC-G---------------CCGCUUGuG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 31508 | 0.66 | 0.765669 |
Target: 5'- aGGUCUUCGgcgaCGaAGC-CGGCGGGCAa -3' miRNA: 3'- aCUAGGAGUa---GC-UCGcGCCGCUUGUg -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 8343 | 0.66 | 0.795391 |
Target: 5'- gGAUUCggCAgcgcCGcGGCgaGCGGCGAACGCa -3' miRNA: 3'- aCUAGGa-GUa---GC-UCG--CGCCGCUUGUG- -5' |
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30689 | 5' | -54.7 | NC_006552.1 | + | 2613 | 0.66 | 0.795391 |
Target: 5'- cGAUCCUgGcUCGAcaaGgGCGcCGAGCGCg -3' miRNA: 3'- aCUAGGAgU-AGCU---CgCGCcGCUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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