Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30690 | 5' | -54.4 | NC_006552.1 | + | 12211 | 0.66 | 0.77032 |
Target: 5'- uUCAUC-GAGCgcgCAGCCugAUGCCCAACc- -3' miRNA: 3'- -AGUGGaCUCG---GUUGG--UACGGGUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 16283 | 0.66 | 0.764136 |
Target: 5'- --uCCUGAGCCcguuucaucccccCCuUGCCCGGCUAc -3' miRNA: 3'- aguGGACUCGGuu-----------GGuACGGGUUGAU- -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 49213 | 0.66 | 0.749516 |
Target: 5'- cCugCUGgAGCgCGGCCugcUGCaCCAACUGg -3' miRNA: 3'- aGugGAC-UCG-GUUGGu--ACG-GGUUGAU- -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 62043 | 0.66 | 0.748462 |
Target: 5'- aCGCCUGAGCCGAcggcgauCCAUccaUCCGGCg- -3' miRNA: 3'- aGUGGACUCGGUU-------GGUAc--GGGUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 51283 | 0.66 | 0.728219 |
Target: 5'- cCugCUGAGCaucgGugCGUGCCaCGACa- -3' miRNA: 3'- aGugGACUCGg---UugGUACGG-GUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 3350 | 0.67 | 0.717417 |
Target: 5'- gCACCaGGGCCAGCUccGCCUGAg-- -3' miRNA: 3'- aGUGGaCUCGGUUGGuaCGGGUUgau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 19339 | 0.67 | 0.706528 |
Target: 5'- aUCACCUGcgGGCgucugAACCAggcgcGCCCAACUc -3' miRNA: 3'- -AGUGGAC--UCGg----UUGGUa----CGGGUUGAu -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 37838 | 0.68 | 0.662337 |
Target: 5'- cCACCcGGGCCAggagccggcagACCAgcagGCCgAACUGu -3' miRNA: 3'- aGUGGaCUCGGU-----------UGGUa---CGGgUUGAU- -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 29359 | 0.68 | 0.662337 |
Target: 5'- -aGCCUGAGCCGccGCCAguugguUGUCCuGCg- -3' miRNA: 3'- agUGGACUCGGU--UGGU------ACGGGuUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 31039 | 0.68 | 0.606519 |
Target: 5'- gCGCCaGAGCCuGGCC--GCCCAGCa- -3' miRNA: 3'- aGUGGaCUCGG-UUGGuaCGGGUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 18090 | 0.69 | 0.584281 |
Target: 5'- cCGCCUG-GCCAGCCccGUCCGGg-- -3' miRNA: 3'- aGUGGACuCGGUUGGuaCGGGUUgau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 10100 | 0.69 | 0.584281 |
Target: 5'- cUCACCaacauagGAGgCAGCCAUGCCUAu--- -3' miRNA: 3'- -AGUGGa------CUCgGUUGGUACGGGUugau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 53551 | 0.69 | 0.573219 |
Target: 5'- gUCGCCgucGCCcuGCCAgagGCCCAGCUu -3' miRNA: 3'- -AGUGGacuCGGu-UGGUa--CGGGUUGAu -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 16689 | 0.71 | 0.476811 |
Target: 5'- -gGCCUGAGCCcGCgc-GCCCAGCa- -3' miRNA: 3'- agUGGACUCGGuUGguaCGGGUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 7112 | 0.71 | 0.446442 |
Target: 5'- -gGCCUGAGCCGACCcacGCCaAGCa- -3' miRNA: 3'- agUGGACUCGGUUGGua-CGGgUUGau -5' |
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30690 | 5' | -54.4 | NC_006552.1 | + | 2478 | 1.07 | 0.001582 |
Target: 5'- uUCACCUGAGCCAACCAUGCCCAACUAc -3' miRNA: 3'- -AGUGGACUCGGUUGGUACGGGUUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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