miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30692 5' -57.3 NC_006552.1 + 2918 1.09 0.000867
Target:  5'- gCAUCGCAGUACUCGCUCCGUCCCGCGg -3'
miRNA:   3'- -GUAGCGUCAUGAGCGAGGCAGGGCGC- -5'
30692 5' -57.3 NC_006552.1 + 48773 0.69 0.486925
Target:  5'- uCAUC-CAGgccGCUCGC-CCaGUCCCGCc -3'
miRNA:   3'- -GUAGcGUCa--UGAGCGaGG-CAGGGCGc -5'
30692 5' -57.3 NC_006552.1 + 46655 0.69 0.486925
Target:  5'- --gCGCuacGUcgUCGC-CCGUCCCGCGg -3'
miRNA:   3'- guaGCGu--CAugAGCGaGGCAGGGCGC- -5'
30692 5' -57.3 NC_006552.1 + 21299 0.68 0.527381
Target:  5'- ---gGCGGUGCUUGCgaaC-UCCCGCGg -3'
miRNA:   3'- guagCGUCAUGAGCGag-GcAGGGCGC- -5'
30692 5' -57.3 NC_006552.1 + 30935 0.67 0.60079
Target:  5'- --gCGCAGUACUUGauggugUCG-CCCGCGu -3'
miRNA:   3'- guaGCGUCAUGAGCga----GGCaGGGCGC- -5'
30692 5' -57.3 NC_006552.1 + 38111 0.67 0.60079
Target:  5'- aCGUUGCGG-ACUCGCaUgCGaUCCGCGa -3'
miRNA:   3'- -GUAGCGUCaUGAGCG-AgGCaGGGCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.