Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30694 | 3' | -60.8 | NC_006552.1 | + | 23381 | 0.66 | 0.475499 |
Target: 5'- --cGCCGucgcccgcacacucuGGGCAuCCAGGGUgCGGCUCg -3' miRNA: 3'- guuCGGC---------------CUCGU-GGUCCCG-GUCGAGg -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 56772 | 0.66 | 0.471614 |
Target: 5'- uCAGGCC---GCGCCAGGcGCgCAGCagUCCc -3' miRNA: 3'- -GUUCGGccuCGUGGUCC-CG-GUCG--AGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 31034 | 0.66 | 0.471614 |
Target: 5'- aGAGCCuGGccgcccAGCAgCAGGGCgCGG-UCCa -3' miRNA: 3'- gUUCGG-CC------UCGUgGUCCCG-GUCgAGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 51876 | 0.66 | 0.461972 |
Target: 5'- --uGCCGGuucGgACUGGGGCCGGgCUgCa -3' miRNA: 3'- guuCGGCCu--CgUGGUCCCGGUC-GAgG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 21207 | 0.66 | 0.461972 |
Target: 5'- --uGCCGcugucGGCgACCAGGGCCAGgCgaaCCu -3' miRNA: 3'- guuCGGCc----UCG-UGGUCCCGGUC-Ga--GG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 53549 | 0.66 | 0.443006 |
Target: 5'- --cGCCGucGCccuGCCAGaGGcCCAGCUUCa -3' miRNA: 3'- guuCGGCcuCG---UGGUC-CC-GGUCGAGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 32494 | 0.66 | 0.443006 |
Target: 5'- --cGCCGGccGGCG-CGGGGCCGGUg-- -3' miRNA: 3'- guuCGGCC--UCGUgGUCCCGGUCGagg -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 13143 | 0.66 | 0.443006 |
Target: 5'- uCGAuCCGGAgGCGCCgAGGGUCgaGGCUgCa -3' miRNA: 3'- -GUUcGGCCU-CGUGG-UCCCGG--UCGAgG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 3825 | 0.66 | 0.433689 |
Target: 5'- gGAGCCGGGccauguuuuggcGCGCCGGcGG-CGGCUacaCCa -3' miRNA: 3'- gUUCGGCCU------------CGUGGUC-CCgGUCGA---GG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 62185 | 0.66 | 0.433689 |
Target: 5'- gGAGCgcgCGGAGCagaucgugcuccACCAGGGCgAGC-Cg -3' miRNA: 3'- gUUCG---GCCUCG------------UGGUCCCGgUCGaGg -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 6846 | 0.66 | 0.424486 |
Target: 5'- --cGCgGGGGCaguuucGCCGuGGCCAGC-CCa -3' miRNA: 3'- guuCGgCCUCG------UGGUcCCGGUCGaGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 15925 | 0.67 | 0.388879 |
Target: 5'- aCGGGCUGcGGCGCUuacguGGGCCuGCaUCCc -3' miRNA: 3'- -GUUCGGCcUCGUGGu----CCCGGuCG-AGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 40462 | 0.67 | 0.380287 |
Target: 5'- -cAGCCG--GCGCC-GGGCCuGCUCg -3' miRNA: 3'- guUCGGCcuCGUGGuCCCGGuCGAGg -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 23817 | 0.68 | 0.36349 |
Target: 5'- -cAGCCGGAaaacagGCGCCacucAGGGUCAGC-Ca -3' miRNA: 3'- guUCGGCCU------CGUGG----UCCCGGUCGaGg -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 38966 | 0.68 | 0.339274 |
Target: 5'- aCAGGUgGcGAaacuGCGCCGGucGGcCCAGCUCCg -3' miRNA: 3'- -GUUCGgC-CU----CGUGGUC--CC-GGUCGAGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 48947 | 0.68 | 0.331466 |
Target: 5'- gGAGCUgGGAGUGCCAGuGGCgCAGUugUCUc -3' miRNA: 3'- gUUCGG-CCUCGUGGUC-CCG-GUCG--AGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 49109 | 0.68 | 0.323791 |
Target: 5'- --uGCuCGGAGCGCUuuGGGGCCgccGGgaCCg -3' miRNA: 3'- guuCG-GCCUCGUGG--UCCCGG---UCgaGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 38288 | 0.69 | 0.301566 |
Target: 5'- --uGCCGGccagcGGCACCAGggaagucguGGCCaucagaAGCUCCu -3' miRNA: 3'- guuCGGCC-----UCGUGGUC---------CCGG------UCGAGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 13957 | 0.69 | 0.280536 |
Target: 5'- gAAGCCGccgauGAGCGCCuGcGCCAGCUUUc -3' miRNA: 3'- gUUCGGC-----CUCGUGGuCcCGGUCGAGG- -5' |
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30694 | 3' | -60.8 | NC_006552.1 | + | 13337 | 0.69 | 0.279856 |
Target: 5'- uCGAGCaagCGGAGCGCCAGcGgcaggaguuggaaGCCAGCaagCCg -3' miRNA: 3'- -GUUCG---GCCUCGUGGUC-C-------------CGGUCGa--GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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