Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30695 | 3' | -62.9 | NC_006552.1 | + | 39778 | 0.68 | 0.276087 |
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Target: 5'- uGCUGCCGUCCCAGCGaacagGGCCa-- -3' miRNA: 3'- gCGGUGGCGGGGUCGCcua--CUGGcgu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 38228 | 0.68 | 0.262535 |
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Target: 5'- gCGUUGCCGUCagGGCGGAuguaguggacaccUGGCCGCAc -3' miRNA: 3'- -GCGGUGGCGGggUCGCCU-------------ACUGGCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 56733 | 0.7 | 0.216363 |
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Target: 5'- uGCC-CUGCUUCAGCaGGAUGuCCGCc -3' miRNA: 3'- gCGGuGGCGGGGUCG-CCUACuGGCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 33490 | 0.7 | 0.207902 |
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Target: 5'- gCGCCACCuGCCCggacuucuuuuccaGGCGGAUGGCaGUAc -3' miRNA: 3'- -GCGGUGG-CGGGg-------------UCGCCUACUGgCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 7935 | 0.7 | 0.200735 |
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Target: 5'- aCGCCcUCGCCgaCGGCGcacGUGACCGCAu -3' miRNA: 3'- -GCGGuGGCGGg-GUCGCc--UACUGGCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 59759 | 0.71 | 0.172411 |
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Target: 5'- gCGCCaguuguucGCCGCgCUGGCGGGUGAUCgGCGg -3' miRNA: 3'- -GCGG--------UGGCGgGGUCGCCUACUGG-CGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 38912 | 0.72 | 0.151589 |
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Target: 5'- cCGUgGCCGCgCCCAGCGGGUagUCGCc -3' miRNA: 3'- -GCGgUGGCG-GGGUCGCCUAcuGGCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 8385 | 0.73 | 0.122992 |
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Target: 5'- cCGCUcgAgCGCgCCCGGCGGAUGGuuguCCGCAg -3' miRNA: 3'- -GCGG--UgGCG-GGGUCGCCUACU----GGCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 5229 | 0.77 | 0.064595 |
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Target: 5'- aCGCa--CGCCCUAGCGGAUGuucACCGCGa -3' miRNA: 3'- -GCGgugGCGGGGUCGCCUAC---UGGCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 3627 | 1.07 | 0.000317 |
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Target: 5'- uCGCCACCGCCCCAGCGGAUGACCGCAu -3' miRNA: 3'- -GCGGUGGCGGGGUCGCCUACUGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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