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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30696 | 5' | -59.3 | NC_006552.1 | + | 4476 | 1.08 | 0.000579 |
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Target: 5'- gGUCACCCGCCGAGUGGUGAAGCCGCAg -3' miRNA: 3'- -CAGUGGGCGGCUCACCACUUCGGCGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 5165 | 0.69 | 0.355514 |
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Target: 5'- -cCACCUGCUGGcgugacGUGGuUGggGCUGCGc -3' miRNA: 3'- caGUGGGCGGCU------CACC-ACuuCGGCGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 5693 | 0.67 | 0.443921 |
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Target: 5'- cUCACCUGCCGGGacacGGUucuuGAGCCgGCAu -3' miRNA: 3'- cAGUGGGCGGCUCa---CCAc---UUCGG-CGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 6597 | 0.66 | 0.50252 |
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Target: 5'- --aGCCCGuuGA-UGGUGGgcgucgguGGCCGCGc -3' miRNA: 3'- cagUGGGCggCUcACCACU--------UCGGCGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 7357 | 0.68 | 0.425252 |
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Target: 5'- -cCACCgGUCGAGUGGUcAAGCUcgaugGCAg -3' miRNA: 3'- caGUGGgCGGCUCACCAcUUCGG-----CGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 13156 | 0.68 | 0.425252 |
|
Target: 5'- -aCACCCGCCgcugucgauccgGAGgcgccgagGGUcGAGGCUGCAc -3' miRNA: 3'- caGUGGGCGG------------CUCa-------CCA-CUUCGGCGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 16639 | 0.67 | 0.463042 |
|
Target: 5'- cUCACUCGCCGAGUcGGcGAAaccCUGCAc -3' miRNA: 3'- cAGUGGGCGGCUCA-CCaCUUc--GGCGU- -5' |
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| 30696 | 5' | -59.3 | NC_006552.1 | + | 25408 | 0.69 | 0.372176 |
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Target: 5'- uGUCGCCCGCa-GGUGGc-AAGCCGUu -3' miRNA: 3'- -CAGUGGGCGgcUCACCacUUCGGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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