Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30697 | 5' | -47.8 | NC_006552.1 | + | 54137 | 0.66 | 0.984783 |
Target: 5'- gCCGggcUUCAGCAGGcUGUAGgCGUGc-- -3' miRNA: 3'- -GGUa--AAGUCGUCC-ACGUUgGUACuuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 3886 | 0.66 | 0.977738 |
Target: 5'- ---gUUCaAGCGGGUGacGCCAUGAGc -3' miRNA: 3'- gguaAAG-UCGUCCACguUGGUACUUu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 57106 | 0.67 | 0.974631 |
Target: 5'- gCCAggUUCAGCAGGUucgugGCGcguuccgGCCAUGc-- -3' miRNA: 3'- -GGUa-AAGUCGUCCA-----CGU-------UGGUACuuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 35939 | 0.67 | 0.960669 |
Target: 5'- gCCAggcUUCagauAGCGGGUGUAcagccaaACCGUGGGAu -3' miRNA: 3'- -GGUa--AAG----UCGUCCACGU-------UGGUACUUU- -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 36564 | 0.68 | 0.946226 |
Target: 5'- uCCAg--CGGCAGGUaacggcggccguagGCAAUCAUGGc- -3' miRNA: 3'- -GGUaaaGUCGUCCA--------------CGUUGGUACUuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 59701 | 0.69 | 0.912737 |
Target: 5'- gCCAUUcucgUAGCGGGUGgAGCCGUa--- -3' miRNA: 3'- -GGUAAa---GUCGUCCACgUUGGUAcuuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 62873 | 0.69 | 0.910695 |
Target: 5'- ---aUUCAGCagcucAGGUGCAgcuuucacgagguaACCAUGAAAc -3' miRNA: 3'- gguaAAGUCG-----UCCACGU--------------UGGUACUUU- -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 33934 | 0.7 | 0.8986 |
Target: 5'- gCCGUUgggggugaUCGGCAGGccgGUAGCCGUGu-- -3' miRNA: 3'- -GGUAA--------AGUCGUCCa--CGUUGGUACuuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 39794 | 0.72 | 0.79054 |
Target: 5'- uCCAguuagCAGCAGGUGCuGCCGUc--- -3' miRNA: 3'- -GGUaaa--GUCGUCCACGuUGGUAcuuu -5' |
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30697 | 5' | -47.8 | NC_006552.1 | + | 4562 | 1.09 | 0.005814 |
Target: 5'- uCCAUUUCAGCAGGUGCAACCAUGAAAg -3' miRNA: 3'- -GGUAAAGUCGUCCACGUUGGUACUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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