Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30698 | 3' | -56.4 | NC_006552.1 | + | 30317 | 0.67 | 0.602761 |
Target: 5'- aGGuCCACcgggAAGCGGGCUugguuGACgCGCAGu -3' miRNA: 3'- aUCuGGUG----UUCGCCCGG-----UUGaGCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 13343 | 0.67 | 0.602761 |
Target: 5'- -uGGCCuucgaGCAAGCGGagcGCCAGCg-GCAGg -3' miRNA: 3'- auCUGG-----UGUUCGCC---CGGUUGagCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 40848 | 0.67 | 0.591842 |
Target: 5'- aAGGCCACAccauGGCaacgaaaGGCCGcauACUCGCAu -3' miRNA: 3'- aUCUGGUGU----UCGc------CCGGU---UGAGCGUc -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 40471 | 0.67 | 0.590752 |
Target: 5'- gUAGGCCACcAGCcggcgccGGGCCuGCUCGg-- -3' miRNA: 3'- -AUCUGGUGuUCG-------CCCGGuUGAGCguc -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 25835 | 0.67 | 0.566873 |
Target: 5'- aUAGGCCgGCAgcGGUacggaaacuugaacGGGuCCGACUCGCGGc -3' miRNA: 3'- -AUCUGG-UGU--UCG--------------CCC-GGUUGAGCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 56933 | 0.68 | 0.537921 |
Target: 5'- cAGGCUACGgucgguGGCGGcGCgGaACUCGCGGu -3' miRNA: 3'- aUCUGGUGU------UCGCC-CGgU-UGAGCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 15105 | 0.68 | 0.527324 |
Target: 5'- --aACCGCAcGCGGGCgGAacgCGCAGc -3' miRNA: 3'- aucUGGUGUuCGCCCGgUUga-GCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 31827 | 0.68 | 0.516806 |
Target: 5'- aGGGCCAgCA--CGGGCCGGCccuUCGUAGg -3' miRNA: 3'- aUCUGGU-GUucGCCCGGUUG---AGCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 5600 | 0.68 | 0.516806 |
Target: 5'- cUGGACCcggcuCAAGCGcaGGCCGcCUgGCAGa -3' miRNA: 3'- -AUCUGGu----GUUCGC--CCGGUuGAgCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 52265 | 0.69 | 0.496032 |
Target: 5'- --cGCCACAAGCucGGCCAGCgCGCuGg -3' miRNA: 3'- aucUGGUGUUCGc-CCGGUUGaGCGuC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 15710 | 0.69 | 0.465606 |
Target: 5'- -uGACCGgugUGAGCGGGCCAAa-CGCAu -3' miRNA: 3'- auCUGGU---GUUCGCCCGGUUgaGCGUc -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 31661 | 0.7 | 0.398669 |
Target: 5'- cUGGugCGCAGGCGcgcagcGGCCGACagcaGCAGg -3' miRNA: 3'- -AUCugGUGUUCGC------CCGGUUGag--CGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 1076 | 0.71 | 0.389619 |
Target: 5'- -cGACCGCAAGCuGuGCCuGAUUCGCAa -3' miRNA: 3'- auCUGGUGUUCGcC-CGG-UUGAGCGUc -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 36385 | 0.71 | 0.354792 |
Target: 5'- -cGACCAgGguGGCGGGuUCAACUCGCu- -3' miRNA: 3'- auCUGGUgU--UCGCCC-GGUUGAGCGuc -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 29548 | 0.73 | 0.299288 |
Target: 5'- gUAGAUCGCAuucaGGUGGGC--GCUCGCGGc -3' miRNA: 3'- -AUCUGGUGU----UCGCCCGguUGAGCGUC- -5' |
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30698 | 3' | -56.4 | NC_006552.1 | + | 4819 | 1.07 | 0.001063 |
Target: 5'- cUAGACCACAAGCGGGCCAACUCGCAGa -3' miRNA: 3'- -AUCUGGUGUUCGCCCGGUUGAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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