Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30699 | 3' | -59.6 | NC_006552.1 | + | 52800 | 0.67 | 0.465417 |
Target: 5'- cGUCGGCGGUgUCGCCUgucACCAGcuuaCGGAa -3' miRNA: 3'- -UAGCCGUUA-GGCGGGa--UGGUCc---GCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 8574 | 0.67 | 0.464445 |
Target: 5'- uUCGGCGGcgcggcuUCUGUCCUGCU--GCGGAa -3' miRNA: 3'- uAGCCGUU-------AGGCGGGAUGGucCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 26826 | 0.67 | 0.446189 |
Target: 5'- -gCaGCAGUUCGCCCaccaggguccgGCCGGcGCGGAa -3' miRNA: 3'- uaGcCGUUAGGCGGGa----------UGGUC-CGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 39258 | 0.67 | 0.427413 |
Target: 5'- cUCGGCAGggacaggaCUGCaCUACCAGGCaccGGAg -3' miRNA: 3'- uAGCCGUUa-------GGCGgGAUGGUCCG---CCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 57645 | 0.67 | 0.426487 |
Target: 5'- -gCGGCGuccuucaGUUCGCCCUGCgCcuGCGGGa -3' miRNA: 3'- uaGCCGU-------UAGGCGGGAUG-GucCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 52368 | 0.67 | 0.418202 |
Target: 5'- gGUUGGCGAcagccuUCUGCUgcgCUGCCcGGCGGGc -3' miRNA: 3'- -UAGCCGUU------AGGCGG---GAUGGuCCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 46191 | 0.68 | 0.382599 |
Target: 5'- cGUCGGCAAaaaacaucUCCGgCUUGCCGGG-GGc -3' miRNA: 3'- -UAGCCGUU--------AGGCgGGAUGGUCCgCCu -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 20397 | 0.68 | 0.365569 |
Target: 5'- -gCGGCAaccgugaaucGUCCGCCCU-CCcGGCGa- -3' miRNA: 3'- uaGCCGU----------UAGGCGGGAuGGuCCGCcu -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 33511 | 0.69 | 0.354784 |
Target: 5'- cGUCGGCcgaGAUCUGCaggcgcgccaCCUGCCcggacuucuuuuccAGGCGGAu -3' miRNA: 3'- -UAGCCG---UUAGGCG----------GGAUGG--------------UCCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 9727 | 0.69 | 0.354784 |
Target: 5'- -cUGGCGAU-CGCCCUGCUgcaccacagcgaagGGGCGGc -3' miRNA: 3'- uaGCCGUUAgGCGGGAUGG--------------UCCGCCu -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 53925 | 0.69 | 0.34907 |
Target: 5'- cGUCGGcCAAUaUgGCCCaUGCC-GGCGGAa -3' miRNA: 3'- -UAGCC-GUUA-GgCGGG-AUGGuCCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 63427 | 0.7 | 0.281514 |
Target: 5'- gGUgGGCGAggCCaacgaGCUCUACCAGuGCGGAa -3' miRNA: 3'- -UAgCCGUUa-GG-----CGGGAUGGUC-CGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 23127 | 0.71 | 0.248684 |
Target: 5'- gGUCGGCGgugaucugcGUCCGCuCCUGcugcuCCAGGCGa- -3' miRNA: 3'- -UAGCCGU---------UAGGCG-GGAU-----GGUCCGCcu -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 770 | 0.72 | 0.207576 |
Target: 5'- cUCGGCAucaucgCCGCCCUcggccugGCCGuGGUGGGg -3' miRNA: 3'- uAGCCGUua----GGCGGGA-------UGGU-CCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 25486 | 0.75 | 0.140103 |
Target: 5'- uGUCGGCGA-CgGCUC-GCCAGGCGGGc -3' miRNA: 3'- -UAGCCGUUaGgCGGGaUGGUCCGCCU- -5' |
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30699 | 3' | -59.6 | NC_006552.1 | + | 5499 | 1.06 | 0.000661 |
Target: 5'- gAUCGGCAAUCCGCCCUACCAGGCGGAc -3' miRNA: 3'- -UAGCCGUUAGGCGGGAUGGUCCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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