Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30699 | 5' | -55.6 | NC_006552.1 | + | 46445 | 0.66 | 0.725418 |
Target: 5'- -gUCCggccugugcaaUGGCCUGUUGCUGCcguGCcGCg -3' miRNA: 3'- aaAGG-----------ACCGGGCGACGAUGcu-UGaCG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 49224 | 0.66 | 0.725418 |
Target: 5'- -cUCCUcggcaGCCUGCUGgaGCGcggccuGCUGCa -3' miRNA: 3'- aaAGGAc----CGGGCGACgaUGCu-----UGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 6998 | 0.66 | 0.724352 |
Target: 5'- --cCCUGGCCgaagccaCGCUGCgcCGggUgGCu -3' miRNA: 3'- aaaGGACCGG-------GCGACGauGCuuGaCG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 22641 | 0.66 | 0.714718 |
Target: 5'- -gUUCgaauGCCCGauaucgaucGCUGCGAACUGCu -3' miRNA: 3'- aaAGGac--CGGGCga-------CGAUGCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 27208 | 0.66 | 0.693082 |
Target: 5'- --gCCUGGUcgauCUGCUGCUG-GAuCUGCu -3' miRNA: 3'- aaaGGACCG----GGCGACGAUgCUuGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 50108 | 0.67 | 0.660212 |
Target: 5'- -cUCCUuaucGGCCCGCUGCggcuucgccaggUucgccuCGAACUGg -3' miRNA: 3'- aaAGGA----CCGGGCGACG------------Au-----GCUUGACg -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 40195 | 0.67 | 0.660212 |
Target: 5'- -cUCCUGGCCaGCcGCU-CGAcaGCgGCg -3' miRNA: 3'- aaAGGACCGGgCGaCGAuGCU--UGaCG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 57988 | 0.67 | 0.660212 |
Target: 5'- -gUCCccGGCgcgcagcgagCCGCUGCcGCGGGcCUGCg -3' miRNA: 3'- aaAGGa-CCG----------GGCGACGaUGCUU-GACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 55161 | 0.67 | 0.627103 |
Target: 5'- -gUCCgcacGGCCUuaccaaGCUGCgg-GAACUGCa -3' miRNA: 3'- aaAGGa---CCGGG------CGACGaugCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 45583 | 0.67 | 0.627103 |
Target: 5'- --aUCUGcGCCUGCUGUacgGCGuuCUGCa -3' miRNA: 3'- aaaGGAC-CGGGCGACGa--UGCuuGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 11188 | 0.67 | 0.605036 |
Target: 5'- --cCCUGGCCCug-GCcGCGAGCcGCc -3' miRNA: 3'- aaaGGACCGGGcgaCGaUGCUUGaCG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 14731 | 0.68 | 0.561254 |
Target: 5'- --cCCUaGGCCCGauCUGggGCGGACUGa -3' miRNA: 3'- aaaGGA-CCGGGC--GACgaUGCUUGACg -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 2611 | 0.68 | 0.550431 |
Target: 5'- -aUCCUGGCUCGacaaggGCgcCGAGCgcgGCa -3' miRNA: 3'- aaAGGACCGGGCga----CGauGCUUGa--CG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 29749 | 0.69 | 0.497432 |
Target: 5'- -gUCUUGcGCguucggCUGCUGCUGCG-ACUGCu -3' miRNA: 3'- aaAGGAC-CG------GGCGACGAUGCuUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 42620 | 0.7 | 0.446844 |
Target: 5'- -aUCCUGGCCCGUcucguagaUGCUgGCGuACcggGCg -3' miRNA: 3'- aaAGGACCGGGCG--------ACGA-UGCuUGa--CG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 41308 | 0.7 | 0.446844 |
Target: 5'- --gCUUGGCaCGCUGCUccucgucauCGAACUGCc -3' miRNA: 3'- aaaGGACCGgGCGACGAu--------GCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 58208 | 0.71 | 0.427421 |
Target: 5'- uUUUgCUGGCCuUGgaGUaUACGAACUGCg -3' miRNA: 3'- -AAAgGACCGG-GCgaCG-AUGCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 17010 | 0.71 | 0.390126 |
Target: 5'- -aUCCUGGUaggCGCcccuuCUACGGACUGCa -3' miRNA: 3'- aaAGGACCGg--GCGac---GAUGCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 46867 | 0.73 | 0.322207 |
Target: 5'- --aCUUGGCgCCGCUGCacccUGGGCUGCu -3' miRNA: 3'- aaaGGACCG-GGCGACGau--GCUUGACG- -5' |
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30699 | 5' | -55.6 | NC_006552.1 | + | 5461 | 1.09 | 0.000903 |
Target: 5'- gUUUCCUGGCCCGCUGCUACGAACUGCu -3' miRNA: 3'- -AAAGGACCGGGCGACGAUGCUUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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