Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30700 | 3' | -57 | NC_006552.1 | + | 15853 | 0.66 | 0.623566 |
Target: 5'- cUGCGcGAcGCUcGCAACCGCUgcaagcgcccauuCGAACg -3' miRNA: 3'- aACGC-CUaCGA-CGUUGGCGG-------------GCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 26939 | 0.66 | 0.635628 |
Target: 5'- cUGCGGAUcgcGCUGCGGCuCG-UUGAGCa -3' miRNA: 3'- aACGCCUA---CGACGUUG-GCgGGCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 2441 | 0.67 | 0.559324 |
Target: 5'- -gGCGGgcGCUGCcGCgccugcgagcgaCGCCUGGACg -3' miRNA: 3'- aaCGCCuaCGACGuUG------------GCGGGCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 36952 | 0.67 | 0.570116 |
Target: 5'- gUUGUGGAUGC-GCgGAUCGC-CGAACa -3' miRNA: 3'- -AACGCCUACGaCG-UUGGCGgGCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 24236 | 0.67 | 0.570116 |
Target: 5'- -gGUGGAUGgUGCcgGACCGCauGGACg -3' miRNA: 3'- aaCGCCUACgACG--UUGGCGggCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 43600 | 0.68 | 0.465606 |
Target: 5'- aUGCGGAUGCacCAGCgCGCCCuGGCc -3' miRNA: 3'- aACGCCUACGacGUUG-GCGGGcUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 8182 | 0.68 | 0.506374 |
Target: 5'- gUGCuGAUGgaGCAucacgACCGgCCGAGCa -3' miRNA: 3'- aACGcCUACgaCGU-----UGGCgGGCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 52373 | 0.69 | 0.440041 |
Target: 5'- cUGCGGGuuggcgacagccuucUGCUGC-GCUGCCCGGc-- -3' miRNA: 3'- aACGCCU---------------ACGACGuUGGCGGGCUuga -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 40334 | 0.69 | 0.44587 |
Target: 5'- aUUGCGGAaGCU-CAuGCUGCUCGAGCa -3' miRNA: 3'- -AACGCCUaCGAcGU-UGGCGGGCUUGa -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 52099 | 0.72 | 0.306789 |
Target: 5'- -gGUGGAagaaGCUGUAGCCGCCUGAc-- -3' miRNA: 3'- aaCGCCUa---CGACGUUGGCGGGCUuga -5' |
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30700 | 3' | -57 | NC_006552.1 | + | 5972 | 1.05 | 0.001162 |
Target: 5'- cUUGCGGAUGCUGCAACCGCCCGAACUg -3' miRNA: 3'- -AACGCCUACGACGUUGGCGGGCUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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