Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30701 | 5' | -58.4 | NC_006552.1 | + | 18886 | 0.66 | 0.611438 |
Target: 5'- aGCGGCCGcucACgGCCGaagaaaagcAGGAgUGCCa -3' miRNA: 3'- -UGCCGGCaa-UGgUGGU---------UCCUgGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 36733 | 0.66 | 0.60079 |
Target: 5'- cGCGauGCCGaUAuCCACCGAGucgaaGCCGCCg -3' miRNA: 3'- -UGC--CGGCaAU-GGUGGUUCc----UGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 56405 | 0.66 | 0.590166 |
Target: 5'- gAUGGUCuugUGCCACC-AGGACaGCCc -3' miRNA: 3'- -UGCCGGca-AUGGUGGuUCCUGgCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 2203 | 0.66 | 0.589106 |
Target: 5'- gGCGGaCCugcGCCGCCAGGuguuggagaaaauGGCCGCCc -3' miRNA: 3'- -UGCC-GGcaaUGGUGGUUC-------------CUGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 55920 | 0.66 | 0.579576 |
Target: 5'- -aGuGCCGUgguagACCACCAAcGGCuCGCCc -3' miRNA: 3'- ugC-CGGCAa----UGGUGGUUcCUG-GCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 16875 | 0.66 | 0.558525 |
Target: 5'- gGCGGCgacCGUUGCUGCCu-GGAauuccgcauaccCCGCCg -3' miRNA: 3'- -UGCCG---GCAAUGGUGGuuCCU------------GGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 29860 | 0.67 | 0.54808 |
Target: 5'- gAUGGCCucgaaucgGUUGCCAgCCgGAGGcuuGCCGCCc -3' miRNA: 3'- -UGCCGG--------CAAUGGU-GG-UUCC---UGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 61617 | 0.67 | 0.537696 |
Target: 5'- cUGGCCGa-GCCGCCGgcgccggcAGGACuUGCCg -3' miRNA: 3'- uGCCGGCaaUGGUGGU--------UCCUG-GCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 42896 | 0.67 | 0.527381 |
Target: 5'- gAUGGuuGccugauCCACCAgcAGGugCGCCg -3' miRNA: 3'- -UGCCggCaau---GGUGGU--UCCugGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 45637 | 0.67 | 0.511035 |
Target: 5'- cCGGCCGcUagcugagcgguguacUGCC-CCAGGGccuucGCCGCCUc -3' miRNA: 3'- uGCCGGC-A---------------AUGGuGGUUCC-----UGGCGGA- -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 3540 | 0.67 | 0.500926 |
Target: 5'- cCGGCCGgacguccaaccugGCCGCCGcacGGACCugGCCg -3' miRNA: 3'- uGCCGGCaa-----------UGGUGGUu--CCUGG--CGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 62551 | 0.68 | 0.467252 |
Target: 5'- gGCGGCCcUU-CUggGCUAGGGACCGCg- -3' miRNA: 3'- -UGCCGGcAAuGG--UGGUUCCUGGCGga -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 31364 | 0.69 | 0.429189 |
Target: 5'- uACGG-CGUgaaACCGCCGAacuGGCCGCCg -3' miRNA: 3'- -UGCCgGCAa--UGGUGGUUc--CUGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 3152 | 0.69 | 0.419961 |
Target: 5'- cCGGCUGgaccUGCUACCcGGaGACUGCCg -3' miRNA: 3'- uGCCGGCa---AUGGUGGuUC-CUGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 1221 | 0.69 | 0.40545 |
Target: 5'- cGCGcGCCaccaacuaccugGCCGCCAAGGGCCugGCCg -3' miRNA: 3'- -UGC-CGGcaa---------UGGUGGUUCCUGG--CGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 7972 | 0.7 | 0.384285 |
Target: 5'- gACcGCCuUUACCGCCAcuGGcACCGCCUc -3' miRNA: 3'- -UGcCGGcAAUGGUGGUu-CC-UGGCGGA- -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 55046 | 0.7 | 0.367217 |
Target: 5'- cGCGGCCGUUGaacgUCACCAGaacaucgcuGACCGCUa -3' miRNA: 3'- -UGCCGGCAAU----GGUGGUUc--------CUGGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 10608 | 0.71 | 0.326873 |
Target: 5'- cACGGCgaagGCCGCCGAGGAuCUGCUg -3' miRNA: 3'- -UGCCGgcaaUGGUGGUUCCU-GGCGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 13004 | 0.71 | 0.304289 |
Target: 5'- -aGGCCug-GCCACCGAGGACUuGCUc -3' miRNA: 3'- ugCCGGcaaUGGUGGUUCCUGG-CGGa -5' |
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30701 | 5' | -58.4 | NC_006552.1 | + | 48852 | 0.77 | 0.119835 |
Target: 5'- uGCGGCCGgcugACCGCCAugcAGaACCGCCg -3' miRNA: 3'- -UGCCGGCaa--UGGUGGU---UCcUGGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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