Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30702 | 3' | -52.3 | NC_006552.1 | + | 64157 | 0.7 | 0.702462 |
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Target: 5'- uGCCgggCAUCacGCAGGCuCAACUCaGGa -3' miRNA: 3'- -CGGaa-GUAGcuUGUCCG-GUUGAGgCC- -5' |
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| 30702 | 3' | -52.3 | NC_006552.1 | + | 33772 | 0.7 | 0.713216 |
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Target: 5'- gGUCUUCGcggugagagUGAcCGGGCCAGCgaUCCGGg -3' miRNA: 3'- -CGGAAGUa--------GCUuGUCCGGUUG--AGGCC- -5' |
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| 30702 | 3' | -52.3 | NC_006552.1 | + | 44136 | 0.7 | 0.734473 |
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Target: 5'- gGCCagcaggUCGUCGAuauuCAGGCCcccaggGGCUuCCGGc -3' miRNA: 3'- -CGGa-----AGUAGCUu---GUCCGG------UUGA-GGCC- -5' |
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| 30702 | 3' | -52.3 | NC_006552.1 | + | 11002 | 0.7 | 0.734473 |
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Target: 5'- cGCCUcacccaaccCAUCGAcACAacGGCCAgcGCUCUGGa -3' miRNA: 3'- -CGGAa--------GUAGCU-UGU--CCGGU--UGAGGCC- -5' |
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| 30702 | 3' | -52.3 | NC_006552.1 | + | 9522 | 0.66 | 0.910379 |
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Target: 5'- -aCUUCAUCGGcugccaggaAgGGGUCGAUcgCCGGg -3' miRNA: 3'- cgGAAGUAGCU---------UgUCCGGUUGa-GGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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