Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30702 | 5' | -55.6 | NC_006552.1 | + | 3357 | 0.66 | 0.785721 |
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Target: 5'- aGCCGGAGCAccaGGGCcagcUCCGccUGAGu -3' miRNA: 3'- cCGGCCUCGUa--CCUGcu--AGGCu-ACUC- -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 24227 | 0.66 | 0.77598 |
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Target: 5'- uGCCGGAccGCAUGGACGuggCCa----- -3' miRNA: 3'- cCGGCCU--CGUACCUGCua-GGcuacuc -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 14494 | 0.66 | 0.773029 |
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Target: 5'- gGGCUGGGGC-UGGaaagucuugguagaGCGcUUCGAUGAa -3' miRNA: 3'- -CCGGCCUCGuACC--------------UGCuAGGCUACUc -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 30436 | 0.66 | 0.766096 |
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Target: 5'- cGCCGGccaggugaaccAGCAUGGACuGGUCCaGGUa-- -3' miRNA: 3'- cCGGCC-----------UCGUACCUG-CUAGG-CUAcuc -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 53241 | 0.67 | 0.683177 |
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Target: 5'- aGGUCGGAGCGcaUGG-CGGUgUGcUGGGg -3' miRNA: 3'- -CCGGCCUCGU--ACCuGCUAgGCuACUC- -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 62274 | 0.68 | 0.618713 |
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Target: 5'- cGCCGGcGGCAUGGuCGAUauucaCGAccgGAGa -3' miRNA: 3'- cCGGCC-UCGUACCuGCUAg----GCUa--CUC- -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 38984 | 0.69 | 0.575832 |
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Target: 5'- uGCCGGucGGCAUGGGCGAca-GGUGGc -3' miRNA: 3'- cCGGCC--UCGUACCUGCUaggCUACUc -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 33342 | 0.7 | 0.533654 |
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Target: 5'- aGGUCGGAGUcgGGGCGcucgCCGAa--- -3' miRNA: 3'- -CCGGCCUCGuaCCUGCua--GGCUacuc -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 55261 | 0.74 | 0.317638 |
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Target: 5'- uGCCGGucuuuugcgaauacGCGUGGGCGAUCCGcagGGGg -3' miRNA: 3'- cCGGCCu-------------CGUACCUGCUAGGCua-CUC- -5' |
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| 30702 | 5' | -55.6 | NC_006552.1 | + | 6322 | 0.98 | 0.007884 |
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Target: 5'- cGGCCGGAG-AUGGACGAUCCGAUGAGc -3' miRNA: 3'- -CCGGCCUCgUACCUGCUAGGCUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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