Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30703 | 3' | -53.2 | NC_006552.1 | + | 36448 | 0.69 | 0.727522 |
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Target: 5'- cGGCGGCGAaggcauGCCGAUgGUGC-CCAcuUgACg -3' miRNA: 3'- -CCGCUGCU------CGGCUA-CAUGuGGU--AgUG- -5' |
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| 30703 | 3' | -53.2 | NC_006552.1 | + | 15453 | 0.69 | 0.727522 |
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Target: 5'- cGGCGAUGcGCCGAU---CACCGUuccCACg -3' miRNA: 3'- -CCGCUGCuCGGCUAcauGUGGUA---GUG- -5' |
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| 30703 | 3' | -53.2 | NC_006552.1 | + | 5731 | 0.68 | 0.738056 |
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Target: 5'- gGGCGGCuGAGCUGcUGgugagGCGCCAcuucggcgcucUCACc -3' miRNA: 3'- -CCGCUG-CUCGGCuACa----UGUGGU-----------AGUG- -5' |
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| 30703 | 3' | -53.2 | NC_006552.1 | + | 37168 | 0.68 | 0.738056 |
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Target: 5'- cGGCGGCGcGCCGGUcuugGU-CACCAccagcuugUCGCc -3' miRNA: 3'- -CCGCUGCuCGGCUA----CAuGUGGU--------AGUG- -5' |
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| 30703 | 3' | -53.2 | NC_006552.1 | + | 62048 | 0.66 | 0.877171 |
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Target: 5'- cGGCGACgccuGAGCCGAcg-GCGauCCAUC-Ca -3' miRNA: 3'- -CCGCUG----CUCGGCUacaUGU--GGUAGuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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