Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30710 | 5' | -60.4 | NC_006552.1 | + | 19776 | 0.66 | 0.45992 |
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Target: 5'- cCGCccGG-GGCC-GGCGCAGCcucccCGGCAa -3' miRNA: 3'- -GCG--UCaCCGGaCCGCGUCGa----GCUGUa -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 16012 | 0.67 | 0.4039 |
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Target: 5'- uGCGGcGGCCUGGUcCGGCUgGuCAg -3' miRNA: 3'- gCGUCaCCGGACCGcGUCGAgCuGUa -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 19493 | 0.68 | 0.320723 |
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Target: 5'- uCGCAGUGaGCCgcacgagugugaUGGuUGCuguGCUCGACAg -3' miRNA: 3'- -GCGUCAC-CGG------------ACC-GCGu--CGAGCUGUa -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 23372 | 0.69 | 0.305708 |
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Target: 5'- cCGCAcacucugGGCauccagGGUGCGGCUCGGCAUu -3' miRNA: 3'- -GCGUca-----CCGga----CCGCGUCGAGCUGUA- -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 46327 | 0.69 | 0.301309 |
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Target: 5'- aGCAGUGcGCUgacGGUGCAcagcgagagccugcuGCUCGGCAUc -3' miRNA: 3'- gCGUCAC-CGGa--CCGCGU---------------CGAGCUGUA- -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 52241 | 0.69 | 0.277308 |
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Target: 5'- gGCcuUGGCCUGGCGguGCguUCGAgGc -3' miRNA: 3'- gCGucACCGGACCGCguCG--AGCUgUa -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 21311 | 0.69 | 0.277308 |
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Target: 5'- uGCGGUGGaCCaGGCGguGCUUG-CGa -3' miRNA: 3'- gCGUCACC-GGaCCGCguCGAGCuGUa -5' |
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| 30710 | 5' | -60.4 | NC_006552.1 | + | 7877 | 1.05 | 0.000641 |
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Target: 5'- gCGCAGUGGCCUGGCGCAGCUCGACAUc -3' miRNA: 3'- -GCGUCACCGGACCGCGUCGAGCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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