Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30713 | 3' | -54 | NC_006552.1 | + | 56340 | 1.09 | 0.001489 |
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Target: 5'- cGUGACCCGAAAGUGGCGCGCAGUAUCc -3' miRNA: 3'- -CACUGGGCUUUCACCGCGCGUCAUAG- -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 8134 | 0.7 | 0.597124 |
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Target: 5'- --cAUCUGcgccAGGUGGuCGCGCAGUAUCg -3' miRNA: 3'- cacUGGGCu---UUCACC-GCGCGUCAUAG- -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 21734 | 0.69 | 0.61923 |
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Target: 5'- -cGACCgGAAGGUauacGGCGCGUAGc--- -3' miRNA: 3'- caCUGGgCUUUCA----CCGCGCGUCauag -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 12306 | 0.68 | 0.663498 |
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Target: 5'- -gGGCCUGAGccaUGGUGCGUAGUcgCu -3' miRNA: 3'- caCUGGGCUUuc-ACCGCGCGUCAuaG- -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 63951 | 0.67 | 0.72878 |
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Target: 5'- aUGcCCCGGAcagcaaucuGGUGGCGCGCGc---- -3' miRNA: 3'- cACuGGGCUU---------UCACCGCGCGUcauag -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 47706 | 0.67 | 0.760256 |
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Target: 5'- ----gCCGGAGGUGGCGCGUAa---- -3' miRNA: 3'- cacugGGCUUUCACCGCGCGUcauag -5' |
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| 30713 | 3' | -54 | NC_006552.1 | + | 57456 | 0.66 | 0.819313 |
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Target: 5'- -aGACUacuuuugcggCGGAGGUGGCGC-CGGUAc- -3' miRNA: 3'- caCUGG----------GCUUUCACCGCGcGUCAUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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